Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat.

Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Research Abstract Details 

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Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Abstract Text:

J Z Li,H Li,T Sasaki,D Holman,B Beres,R J Dumont,D D Pittman,G R Hankins,G A Helm,J Z Li,H Li,T Sasaki,D Holman,B Beres,R J Dumont,D D Pittman,G R Hankins,G A Helm,

Bone morphogenetic protein (BMP) adenoviral vectors for the induction of osteogenesis are being developed for the treatment of bone pathology. However, it is still unknown which BMP adenoviral vector has the highest potential to stimulate bone formation in vivo. In this study, the osteogenic activities of recombinant human BMP-2, BMP-4, BMP-6, BMP-7, and BMP-9 adenoviruses were compared in vitro, in athymic nude rats, and in Sprague-Dawley rats. In vitro osteogenic activity was assessed by measuring the alkaline phosphatase activity in C2C12 cells transduced by the various BMP vectors. The alkaline phosphatase activity induced by 2 x 10(5) PFU/well of BMP viral vector was 4890 x 10(-12) U/well for ADCMVBMP-9, 302 x 10(-12) U/well for ADCMVBMP-4, 220 x 10(-12) U/well for ADCMVBMP-6, 45 x 10(-12) U/well for ADCMVBMP-2, and 0.43 x 10(-12) U/well for ADCMVBMP-7. The average volume of new bone induced by 10(7) PFU of BMP vector in athymic nude rats was 0.37+/-0.03 cm(3) for ADCMVBMP-2, 0.89+/-0.07 cm(3) for ADCMVBMP-4, 1.02+/-0.07 cm(3) for ADCMVBMP-6, 0.24+/-0.05 cm(3) for ADCMVBMP-7, and 0.63+/-0.07 cm(3) for ADCMVBMP-9. In immunocompetent Sprague-Dawley rats, no bone formation was demonstrated in the ADCMVBMP-2, ADCMVBMP-4, and ADCMVBMP-7 groups. ADCMVBMP-6 at a viral dose of 10(8) PFU induced 0.10+/-0.03 cm(3) of new bone, whereas ADCMVBMP-9 at a lower viral dose of 10(7) PFU induced more bone, with an average volume of 0.29+/-0.01 cm(3).

Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Publishing Authors By Initials

JZ Li,H Li,T Sasaki,D Holman,B Beres,RJ Dumont,DD Pittman,GR Hankins,GA Helm,JZ Li,H Li,T Sasaki,D Holman,B Beres,RJ Dumont,DD Pittman,GR Hankins,GA Helm,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Gene therapy

VOLUME: 10

Page Numbers: 1735-43

Journal Abbreviation: Gene Ther.

ISSN: 0969-7128

DAY: 19

MONTH: Sep

YEAR: 2003

Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Information

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LANGUAGE: eng

NlmUniqueID: 9421525

Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: methods

Chemical & Substance for Abstract: Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat.

AFFILIATION: Molecular Neurosurgery Lab, Department of Neurosurgery, University of Virginia Health System, Charlottesville, VA 22908, USA.

Country: England

AGENCY: United States NIAMS

GRANT: R01 AR46488-01A2

ACRONYM: AR

MEDLINETA: Gene Ther

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