Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2).

Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). Research Abstract Details 

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Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). Abstract Text:

Kazuharu Irie,Cansu Alpaslan,Koichiro Takahashi,Yukari Kondo,Naoya Izumi,Yasunori Sakakura,Eichi Tsuruga,Tamio Nakajima,Sadakazu Ejiri,Hidehiro Ozawa,Toshihiko Yajima,

Osteoclast differentiation in the process of ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2) was examined to clarify the relationship between osteoclast development and rhBMP-2-induced bone formation. A combination of rhBMP-2 with a porous microsphere (PMS) and blood clot was implanted subcutaneously on the bilateral chest muscles of rats. Tartrate-resistant acid phosphatase (TRAPase) activity, cathepsin K (cath K), and calcitonin receptor (CTR), as markers of osteoclasts and their precursors, were examined using enzyme and immunohistochemical analysis up to 7 days after implantation. Mononuclear cells positive for TRAPase, cath K, and CTR first appeared on day 3 in connective tissue surrounding the PMS after implantation of rhBMP-2. Simultaneously, alkaline phosphatase activity became detectable in mesenchymal cells in the connective tissue. Electron microscopy demonstrated some mononuclear cells with abundant mitochondria and poorly developed rough endoplasmic reticulum in the proximity of mesenchymal cells. However, there was no evidence of cartilage or bone matrix formation on day 3. Osteoclasts in various stages of development, classified by the pattern of immunoreactivity for cath K, were observed by day 7. The polarized intracellular distribution of cath K was found only in osteoclasts attached to bone matrix. In conclusion, we have demonstrated for the first time the appearance of osteoclast precursors before bone matrix formation induced by rhBMP-2, suggesting that bone matrix is not a prerequisite for osteoclast precursor recruitment. Furthermore, we suggest that differentiation into polarized functional osteoclasts is accomplished when the osteoclasts attach to the bone matrix.

Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). Publishing Authors By Initials

K Irie,C Alpaslan,K Takahashi,Y Kondo,N Izumi,Y Sakakura,E Tsuruga,T Nakajima,S Ejiri,H Ozawa,T Yajima,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bone and mineral metabolism

VOLUME: 21

Page Numbers: 363-9

Journal Abbreviation: J. Bone Miner. Metab.

ISSN: 0914-8779

DAY: 19

MONTH: 11

YEAR: 2003

Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). Information

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LANGUAGE: eng

NlmUniqueID: 9436705

Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2). Information

Substance Name: recombinant human bone morphogenetic pro

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Grant and Affiliation Information for Osteoclast differentiation in ectopic bone formation induced by recombinant human bone morphogenetic protein 2 (rhBMP-2).

AFFILIATION: Department of Oral Anatomy, Health Sciences University of Hokkaido School of Dentistry, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.

Country: Japan

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MEDLINETA: J Bone Miner Metab

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