Osteoblast adhesion and morphology on TiO(2) depends on the competitive preadsorption of albumin and fibronectin.

Osteoblast adhesion and morphology on TiO(2) depends on the competitive preadsorption of albumin and fibronectin. Research Abstract Details 

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Osteoblast adhesion and morphology on TiO(2) depends on the competitive preadsorption of albumin and fibronectin. Abstract Text:

S R Sousa,M Lamghari,P Sampaio,P Moradas-Ferreira,M A Barbosa,S R Sousa,M Lamghari,P Sampaio,P Moradas-Ferreira,M A Barbosa,

This study aims at assessing the influence of the competitive preadsorption of human serum albumin (HSA) and human plasma fibronectin (FN) from binary solutions and 10% plasma on MC3T3-E1 osteoblast adhesion and morphology on two types of TiO(2) substrates. One was commercially pure titanium with a titanium oxide layer formed in an H(2)O(2) solution and the other TiO(2) sputtered on Si (Sousa et al., Langmuir 2004; 20:9745-9754.). The strategy applied in the present investigation was to compare osteoblast adhesion to surfaces preadsorbed with HSA, FN, HSA/FN = 1, HSA/FN = 200, and 10% plasma. The adsorption of proteins was evaluated measuring the amount and the effectiveness of binding with radiolabeled proteins, (125)I-FN and (125)I-HSA. Our results indicated that MC3T3-E1 osteoblast adhesion correlates well with the amounts of FN and HSA adsorbed on TiO(2) surfaces. Also, we found that fewer osteoblasts adhered to both substrates preadsorbed with HSA, HSA/FN = 200, and 10% plasma, after 4 and 24 h, than to the surfaces preadsorbed with FN and HSA/FN = 1. For the latter, FN was able to compensate the inhibitory effect of HSA on osteoblast adhesion. Therefore, the presence of lower amounts of coadsorbed albumin may improve presentation of FN in a more integrin-recognized conformation, suggesting that some degree of molecular packing prevents loss of integrin-binding activity. FN reversibility does not seem to be dependent on the HSA/FN adsorption mass ratio in solution, suggesting that FN competitively adsorbs to TiO(2) in a favorable conformation and does not suffers subsequent conformational changes allowing exchange with other FN molecules in solution. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008.

Osteoblast adhesion and morphology on TiO(2) depends on the competitive preadsorption of albumin and fibronectin. Publishing Authors By Initials

SR Sousa,M Lamghari,P Sampaio,P Moradas-Ferreira,MA Barbosa,SR Sousa,M Lamghari,P Sampaio,P Moradas-Ferreira,MA Barbosa,

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Osteoblast adhesion and morphology on TiO(2) depends on the competitive preadsorption of albumin and fibronectin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part A

VOLUME: 84

Page Numbers: 281-90

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ISSN: 1549-3296

DAY: 20

MONTH: Feb

YEAR: 2008

Osteoblast adhesion and morphology on TiO(2) depends on the competitive preadsorption of albumin and fibronectin. Information

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LANGUAGE: eng

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AFFILIATION: INEB - Instituto de Engenharia Biomédica, Laboratório de Biomaterials, R. do Campo Alegre, 823, 4150-180 Porto, Portugal.

Country: United States

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MEDLINETA: J Biomed Mater Res A

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