Organized unidirectional waves of ATP hydrolysis within a RecA filament.

Organized unidirectional waves of ATP hydrolysis within a RecA filament. Research Abstract Details 

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Organized unidirectional waves of ATP hydrolysis within a RecA filament. Abstract Text:

Julia M Cox,Oleg V Tsodikov,Michael M Cox,

The RecA protein forms nucleoprotein filaments on DNA, and individual monomers within the filaments hydrolyze ATP. Assembly and disassembly of filaments are both unidirectional, occurring on opposite filament ends, with disassembly requiring ATP hydrolysis. When filaments form on duplex DNA, RecA protein exhibits a functional state comparable to the state observed during active DNA strand exchange. RecA filament state was monitored with a coupled spectrophotometric assay for ATP hydrolysis, with changes fit to a mathematical model for filament disassembly. At 37 degrees C, monomers within the RecA-double-stranded DNA (dsDNA) filaments hydrolyze ATP with an observed k(cat) of 20.8 +/- 1.5 min(-1). Under the same conditions, the rate of end-dependent filament disassembly (k(off)) is 123 +/- 16 monomers per minute per filament end. This rate of disassembly requires a tight coupling of the ATP hydrolytic cycles of adjacent RecA monomers. The relationship of k(cat) to k(off) infers a filament state in which waves of ATP hydrolysis move unidirectionally through RecA filaments on dsDNA, with successive waves occurring at intervals of approximately six monomers. The waves move nearly synchronously, each one transiting from one monomer to the next every 0.5 s. The results reflect an organization of the ATPase activity that is unique in filamentous systems, and could be linked to a RecA motor function.

Organized unidirectional waves of ATP hydrolysis within a RecA filament. Publishing Authors By Initials

JM Cox,OV Tsodikov,MM Cox,

For similar enzymes and coenzymes: enzymes: recombinases: rec a recombinases research abstracts see: enzymes and coenzymes: enzymes: recombinases: rec a recombinases research

PUBMED ID PMID:

MEDLINE DATE:

Organized unidirectional waves of ATP hydrolysis within a RecA filament. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: PLoS biology

VOLUME: 3

Page Numbers: e52

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 8

MONTH: 02

YEAR: 2005

Organized unidirectional waves of ATP hydrolysis within a RecA filament. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Organized unidirectional waves of ATP hydrolysis within a RecA filament. Keywords Mesh Terms:

KEYWORDS: Rec A Recombinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Organized unidirectional waves of ATP hydrolysis within a RecA filament. Information

Substance Name: Rec A Recombinases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Organized unidirectional waves of ATP hydrolysis within a RecA filament.

AFFILIATION: Department of Biochemistry, University of Wisconsin, Madison, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM32335

ACRONYM: GM

MEDLINETA: PLoS Biol

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