Organelle redox of CF and CFTR-corrected airway epithelia.

Organelle redox of CF and CFTR-corrected airway epithelia. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Organelle redox of CF and CFTR-corrected airway epithelia. Abstract Text:

Christian Schwarzer,Beate Illek,Jung H Suh,S James Remington,Horst Fischer,Terry E Machen,

In cystic fibrosis reduced CFTR function may alter redox properties of airway epithelial cells. Redox-sensitive GFP (roGFP1) and imaging microscopy were used to measure the redox potentials of the cytosol, endoplasmic reticulum (ER), mitochondria, and cell surface of cystic fibrosis nasal epithelial cells and CFTR-corrected cells. We also measured glutathione and cysteine thiol redox states in cell lysates and apical fluids to provide coverage over a range of redox potentials and environments that might be affected by CFTR. As measured with roGFP1, redox potentials at the cell surface (approx -207+/-8 mV) and in the ER (approx -217+/-1 mV) and rates of regulation of the apical fluid and ER lumen after DTT treatment were similar for CF and CFTR-corrected cells. CF and CFTR-corrected cells had similar redox potentials in mitochondria (-344+/-9 mV) and cytosol (-322+/-7 mV). Oxidation of carboxydichlorodihydrofluorescein diacetate and of apical Amplex red occurred at equal rates in CF and CFTR-corrected cells. Glutathione and cysteine redox couples in cell lysates and apical fluid were equal in CF and CFTR-corrected cells. These quantitative estimates of organelle redox potentials combined with apical and cell measurements using small-molecule couples confirmed there were no differences in the redox properties of CF and CFTR-corrected cells.

Organelle redox of CF and CFTR-corrected airway epithelia. Publishing Authors By Initials

C Schwarzer,B Illek,JH Suh,SJ Remington,H Fischer,TE Machen,

For similar respiratory system: respiratory mucosa research abstracts see: respiratory system: respiratory mucosa research

PUBMED ID PMID:

MEDLINE DATE:

Organelle redox of CF and CFTR-corrected airway epithelia. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Free radical biology & medicine

VOLUME: 43

Page Numbers: 300-16

Journal Abbreviation: Free Radic. Biol. Med.

ISSN: 0891-5849

DAY: 29

MONTH: 04

YEAR: 2007

Organelle redox of CF and CFTR-corrected airway epithelia. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8709159

Organelle redox of CF and CFTR-corrected airway epithelia. Keywords Mesh Terms:

KEYWORDS: Respiratory Mucosa

MESH TERMS: physiopathology

Chemical & Substance for Abstract: Organelle redox of CF and CFTR-corrected airway epithelia. Information

Substance Name: Glutathione

Registry Number: 70-18-8

Grant and Affiliation Information for Organelle redox of CF and CFTR-corrected airway epithelia.

AFFILIATION: Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, USA.

Country: United States

AGENCY: United States NCCAM

GRANT: P01 AT002620

ACRONYM: AT

MEDLINETA: Free Radic Biol Med

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Organelle redox of CF and CFTR-corrected airway epithelia Related Publications

• Effects of cystic fibrosis transmembrane conductance regulator and DeltaF508CFTR on inflammatory response, ER stress, and Ca2+ of airway epithelia.
• Flagellin-stimulated Cl- secretion and innate immune responses in airway epithelia: role for p38.
• Organelle redox of CF and CFTR-corrected airway epithelia.
• Redox-independent Activation of NF-{kappa}B by Pseudomonas aeruginosa Pyocyanin in a Cystic Fibrosis Airway Epithelial Cell Line.
• Role of Ca2+ in responses of airway epithelia to Pseudomonas aeruginosa, flagellin, ATP, and thapsigargin.
• We can be leaders in addressing children's oral healthcare needs.