Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform.

Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. Abstract Text:

S Tsuboi,T Suga,K Takishima,G Mamiya,K Matsui,Y Ozeki,M Yamada,

Four kinds of nonspecific lipid transfer proteins (nsLTP) were purified from different organs of castor bean (Ricinus communis L.) seedlings. Amino acid compositions and amino-terminal sequences of the four nsLTPs were determined and compared with those of castor bean isoforms, nsLTP-A, -B, and -C, previously reported [Takishima et al. (1986) Biochim. Biophys. Acta 870, 248-255; Takishima et al. (1988) Eur. J. Biochem. 177, 241-249]. Two isoforms from the cotyledons were identified as nsLTP-A and -C, one isoform from the endosperms as nsLTP-B, and the other was a new isoform from the axes. This new isoform was named nsLTP-D and its amino acid sequence was determined. These results demonstrated organ-specific occurrence of the nsLTP isoforms in castor bean seedlings. The isoforms nsLTP-A, -B, -C, and -D showed similar transfer activity not only for phosphatidylcholine and phosphatidylethanolamine but also for monogalactosyldiacylglycerol, although the homology among their amino acid sequences ranged from 70 to 30%. Two cDNA clones (pnsLTP-C and pnsLTP-D) for nsLTPs of castor bean seedlings were isolated and sequenced. pnsLTP-C was the cDNA clone for nsLTP-C expressed in the cotyledons, and pnsLTP-D was that for nsLTP-D in the axis. A coupled in vitro transcription-translation analysis of both cDNA clones revealed that pnsLTP-C encodes the full-length of nsLTP-C precursor (pro-nsLTP-C), while pnsLTP-D encodes a part of nsLTP-D precursor. PronsLTP-C contained a 24-amino acid pre-sequence preceding the mature nsLTP-C (92 amino acids).(ABSTRACT TRUNCATED AT 250 WORDS)

Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. Publishing Authors By Initials

S Tsuboi,T Suga,K Takishima,G Mamiya,K Matsui,Y Ozeki,M Yamada,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 110

Page Numbers: 823-31

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1991

Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform.

AFFILIATION: Department of Biology, College of Arts and Sciences, University of Tokyo.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: S78751

Number Hits: 0

Organ-specific occurrence and expression of the isoforms of nonspecific lipid transfer protein in castor bean seedlings, and molecular cloning of a full-length cDNA for a cotyledon-specific isoform Related Publications

• A classification method of hand movements using multi channel electrode.
• A method for measurement of water temperature in micro-region using near infrared light.
• A phase II trial of thalidomide in patients with refractory endometrial cancer and correlation with angiogenesis biomarkers: a Gynecologic Oncology Group study.
• Anterior transpetrosal approach to the prepontine epidermoids.
• Assessing health disparities in rural Hawaii using the Hoshin facilitation method.
• Binding activities by propiverine and its N-oxide metabolites of L-type calcium channel antagonist receptors in the rat bladder and brain.
• Chemical Properties and Anti-Complementary Activities of Polysaccharide Fractions from Roots and Leaves of Panax ginseng.
• Developmental transition of EEG spectra to alpha band in a sample of children with developmental disabilities.
• Effects of Exogenous Polyamines on Tropane Alkaloid Production by a Root Culture of Duboisia myoporoides.
• Flow Cytometric Analysis of Cellular Berberine Contents in High- and Low-Producing Cell Lines of Coptis japonica Obtained by Repeated Selection.
• Gas chromatography-mass spectrometric study of 19-oxygenation of the aromatase inhibitor 19-methylandrostenedione with human placental microsomes.
• High concentrations of D-amino acids in human gastric juice.
• Influences of body posture on duration of oral swallowing in normal young adults.
• Influences of thermal and gustatory characteristics on sensory and motor aspects of swallowing.
• Inhibition of tyrosine activity by cultured lichen tissues and bionts.
• Insufficiency fracture in the medial wall of the acetabulum after total hip arthroplasty.
• Lipopolysaccharide-independent limulus amebocyte lysate activating, mitogenic and anti-complementary activities of pectic polysaccharides from chinese herbs.
• Long-term grooming partnerships between unrelated adult females in a free-ranging group of Japanese monkeys (Macaca fuscata).
• Pathology of renal xenograft rejection in pig to non-human primate transplantation.
• Presumed stromal graft rejection after automated lamellar therapeutic keratoplasty: case report.
• STAT3 and PKC differentially regulate telomerase activity during megakaryocytic differentiation of K562 cells.
• Scopolamine Production in Suspension Cultures and Redifferentiated Roots of Hyoscyamus niger.
• Serial assessment of left ventricular function in various patient groups with Tl-201 gated myocardial perfusion SPECT.
• Stimulating effect of Japanese herbal (kampo) medicine, hochuekkito on upper respiratory mucosal immune system.
• The Smallest Electron Storage Ring for High-Intensity Far-Infrared and Hard X-ray Productions.
• Three-dimensional assessment of urinary stone on non-contrast helical computed tomography as the predictor of stonestreet formation after extracorporeal shock wave lithotripsy for stones smaller than 20 mm.
• Thymic rejuvenation and the induction of tolerance by adult thymic grafts.
• Transbronchial localization of small pulmonary lesions for thoracoscopic resection.
• Usefulness of serum protein profiling for prediction of preoperative chemoradiosensitivity of esophageal cancer.
• [In vivo transduction of EGFP into female mouse reproductive system by electroporation and microbubble-enhanced sonoporation]