Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats.

Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats. Research Abstract Details 

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Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats. Abstract Text:

Jianhua Hou,Ruixiang Yan,Dongfeng Ding,Liquan Yang,Cuiyan Wang,Zhiqiang Wu,Xiaoju Yu,Weidong Li,Minggang Li,Jianhua Hou,Ruixiang Yan,Dongfeng Ding,Liquan Yang,Cuiyan Wang,Zhiqiang Wu,Xiaoju Yu,Weidong Li,Minggang Li,

Glucagon-like peptide-1 (7-36) amide (GLP-1), a gut hormone released into the blood stream after feeding, can stimulate insulin secretion by potentiating the insulinotropic action of glucose. An expression vector pET-22bG8, encoding a fusion protein containing eight tandem repeat GLP-1 ([Ser(8), Gln(26), Asp(34)]-GLP-1) analogues, was constructed and transformed into the Escherichia coli BL21(DE3) strain over-expressing the His-tagged fusion protein under the IPTG promoter. SDS-PAGE and Western blot analysis demonstrated that the His-tagged GLP-1 fusion protein migrated as a single protein with a molecular weight of 32 kDa. Following chronic (10 days) oral administration (20 mg kg(-1) day(-1)) of the fusion protein to diabetic rats, serum glucose levels were significantly lowered from 26 +/- 2.5 to 7.9 +/- 1.4 mmol/l. Further studies are needed to evaluate the potential use for GLP-1 analogue short peptide in the treatment of diabetes mellitus.

Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats. Publishing Authors By Initials

J Hou,R Yan,D Ding,L Yang,C Wang,Z Wu,X Yu,W Li,M Li,J Hou,R Yan,D Ding,L Yang,C Wang,Z Wu,X Yu,W Li,M Li,

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Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biotechnology letters

VOLUME: 29

Page Numbers: 1439-46

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 21

MONTH: 06

YEAR: 2007

Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats. Information

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LANGUAGE: eng

NlmUniqueID: 8008051

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Grant and Affiliation Information for Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats.

AFFILIATION: The Key Laboratory for Bioactivematerial of Ministry of Education, Institute for Molecular Biology, College of Life Science, Nankai University, Tianjin, 300071, P.R. China.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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