Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry.

Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry. Research Abstract Details 

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Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry. Abstract Text:

Greg T Cantin,Teresa R Shock,Sung Kyu Park,Hiten D Madhani,John R Yates,

An automated online multidimensional liquid chromatography system coupled to ESI-based tandem mass spectrometry was used to assess the effectiveness of TiO2 in the enrichment of phosphopeptides from tryptic digests of protein mixtures. By monitoring the enrichment of phosphopeptides, an optimized set of loading, wash, and elution conditions were realized for TiO2. A comparison of TiO2 with other resins used for phosphopeptide enrichment, Fe(III)-IMAC and ZrO2, was also carried out using tryptic digests of both simple and moderately complex protein mixtures; where TiO2 was shown to be superior in performance.

Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry. Publishing Authors By Initials

GT Cantin,TR Shock,SK Park,HD Madhani,JR Yates,

For similar inorganic chemicals: elements: metals, heavy: zirconium research abstracts see: inorganic chemicals: elements: metals, heavy: zirconium research

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Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 4666-73

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 25

MONTH: 05

YEAR: 2007

Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Zirconium

MESH TERMS: chemistry

Chemical & Substance for Abstract: Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry.

AFFILIATION: Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, SR11, La Jolla, California 92037, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41 RR11823-10

ACRONYM: RR

MEDLINETA: Anal Chem

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