Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome.

Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome. Research Abstract Details 

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Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome. Abstract Text:

Lesley A Kane,Christina K Yung,Giulio Agnetti,Irina Neverova,Jennifer E Van Eyk,

Separation of basic proteins with 2-DE presents technical challenges involving protein precipitation, load limitations, and streaking. Cardiac mitochondria are enriched in basic proteins and difficult to resolve by 2-DE. We investigated two methods, cup and paper bridge, for sample loading of this subproteome into the basic range (pH 6-11) gels. Paper bridge loading consistently produced improved resolution of both analytical and preparative protein loads. A unique benefit of this technique is that proteins retained in the paper bridge after loading basic gels can be reloaded onto lower pH gradients (pH 4-7), allowing valued samples to be analyzed on multiple pH ranges.

Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome. Publishing Authors By Initials

LA Kane,CK Yung,G Agnetti,I Neverova,JE Van Eyk,

For similar cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: mitochondria: submitochondrial particles research abstracts see: cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: mitochondria: submitochondrial particles research

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Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proteomics

VOLUME: 6

Page Numbers: 5683-7

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 3

MONTH: Nov

YEAR: 2006

Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome. Information

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LANGUAGE: eng

NlmUniqueID: 101092707

Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome. Keywords Mesh Terms:

KEYWORDS: Submitochondrial Particles

MESH TERMS: chemistry

Chemical & Substance for Abstract: Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Optimization of paper bridge loading for 2-DE analysis in the basic pH region: application to the mitochondrial subproteome.

AFFILIATION: Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21224, USA.

Country: Germany

AGENCY: United States NHLBI

GRANT: P01HL081427

ACRONYM: HL

MEDLINETA: Proteomics

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