Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo.

Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo. Research Abstract Details 

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Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo. Abstract Text:

Ying Zhang,Wei Wang,Yubing Xie,Weiting Yu,Guojun Lv,Xin Guo,Ying Xiong,Xiaojun Ma,Ying Zhang,Wei Wang,Yubing Xie,Weiting Yu,Guojun Lv,Xin Guo,Ying Xiong,Xiaojun Ma,Ying Zhang,Wei Wang,Yubing Xie,Weiting Yu,Guojun Lv,Xin Guo,Ying Xiong,Xiaojun Ma,

Microencapsulation of recombinant cells secreting endostatin offers a promising approach to tumor gene therapy in which therapeutic protein is delivered in a sustainable and long-term fashion by encapsulated recombinant cells. However, the studies of cell growth and protein production in vivo are very limited. In this study, the effects of microencapsulation parameters on in vivo cell growth, endostatin production, and microcapsule stability after implantation in the peritoneal cavity of mice were for the first time investigated. Microcapsules with liquid core reached higher cell density and endostatin production at day 18 than microcapsules with solid core. There was no significant difference in stability whether the core of the microcapsule was solid or liquid. Decrease in microcapsule size increased the stability of microcapsule. The microcapsules kept intact in the peritoneal cavity of mice after 36 days of implantation when the microcapsules size was 240 mum in diameter, which gave rise to high endostatin production as well. The optimized microencapsulation conditions for in vivo implantation are liquid core and 240 mum in diameter. This study provides useful information for antiangiogenic gene therapy to tumors using microencapsulated recombinant cells. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008.

Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo. Publishing Authors By Initials

Y Zhang,W Wang,Y Xie,W Yu,G Lv,X Guo,Y Xiong,X Ma,Y Zhang,W Wang,Y Xie,W Yu,G Lv,X Guo,Y Xiong,X Ma,Y Zhang,W Wang,Y Xie,W Yu,G Lv,X Guo,Y Xiong,X Ma,

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Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part B,

VOLUME: 84

Page Numbers: 79-88

Journal Abbreviation: J. Biomed. Mater. Res. Part B

ISSN: 1552-4973

DAY: 11

MONTH: Jan

YEAR: 2008

Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo. Information

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LANGUAGE: eng

NlmUniqueID: 101234238

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AFFILIATION: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese, Academy of Sciences, Dalian 116023, People's Republic of China.

Country: United States

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MEDLINETA: J Biomed Mater Res B Appl Biom

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