Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology.

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Abstract Text:

Y-H Wang,J-T Feng,Q Zhang,X Zhang,

AIMS: To evaluate the influence of environmental parameters on the production of antibiotics (xenocoumacins and nematophin) by Xenorhabdus nematophila and enhance the antibiotic activity. METHODS AND RESULTS: Response surface methodology (RSM) was employed to study the effects of five parameters (the initial pH, medium volume in flask, rotary speed, temperature and inoculation volume) on the production of antibiotics in flask cultures by X. nematophila YL001. A 2(5-1)-factorial central composite design was chosen to explain the combined effects of the five parameters and to design a minimum number of experiments. The experimental results and software-predicted values of production of antibiotics were comparable. The statistical analysis of the results showed that, in the range studied, medium volume in flask, rotary speed, temperature and inoculation volume had a significant effect (P < 0.05) on the production of antibiotics at their individual level, medium volume in flask and rotary speed showed a significant influence at interactive level and were most significant at individual level. The maximum antibiotic activity was achieved at the initial pH 7.64, medium volume in 250 ml flask 25 ml, rotary speed of 220 rev min(-1), temperature 27.8 degrees C and inoculation volume of 15.0%. Maximum antibiotic activity of 331.7 U ml(-1) was achieved under the optimized condition. CONCLUSIONS: As far as known, there are no reports of production of antibiotic from X. nematophila by engineering the condition of fermentation using RSM. The results strongly support the use of RSM for fermentation condition optimization. The optimization of the environmental parameters resulted not only in a 43.4% higher antibiotic activity than unoptimized conditions but also in a reduced amount of the experiments. The chosen method of optimization of fermentation condition was efficient, relatively simple and time and material saving. SIGNIFICANCE AND IMPACT OF THE STUDY: This study should contribute towards improving the antibiotics activity of X. nematophila. Integrated into a broader study of the impact of environmental factors on the production of antibiotic, this work should help to build more rational control strategy, possibly involving scale-up of production of antibiotics by X. nematophila.

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Publishing Authors By Initials

YH Wang,JT Feng,Q Zhang,X Zhang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of applied microbiology

VOLUME: 104

Page Numbers: 735-44

Journal Abbreviation: J. Appl. Microbiol.

ISSN: 1365-2672

DAY: 22

MONTH: 10

YEAR: 2007

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9706280

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology.

AFFILIATION: Biorational Pesticides Research and Service Center, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, China.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Appl Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology Related Publications

• A comparative study on the intercuspal occlusion among TMD patients, malocclusion patients and university students.
• Effect of retinoblastoma gene on human osteosarcoma cell line OS732.
• Enhanced antioxidant and cytoprotective abilities of vitamin E succinate is associated with a rapid uptake advantage in rat hepatocytes and mitochondria.
• Intervention by azithromycin on bleomycin-induced lung injury in rats and its mechanisms.
• Method development of adsorbed stationary phase open tubular capillary electrochromatography.
• Supercritical fluid CO2 extraction of colchicine from colchicine Autumnele liliaceae.
• Vascular endothelial growth factor gene expression in patients' ischemic skeletal muscle with diabetic foot.
• [A case of hypodermiasis with empyema]
• [Analysis of hepatitis G virus genotypes in some areas of China]
• [Autologous dendritic cells eliciting cytotoxicity of bone marrow cells against chronic granulocytic leukemia]
• [Changes of human recombination bone morphogenetic protein-2 in bone and marrow in tail suspended rats]
• [Cloning of the mouse Doc-1R gene by genomic walking]
• [Construction of recombinant adenoviruses carrying human urokinase-type plasminogen activator and its expression in hepatic stellate cells in vitro]
• [Effect of inhibition of telomerase activity on cisplatin-induced apoptosis in K562 cells]
• [Effects of electrical stimulation of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats]
• [Fatal complications in traffic accident injury]
• [Indomethacin induces apoptosis of K562 cells through activation of caspases and elevation of intracellular free calcium]
• [Infection and replication of hepatitis B virus and its clearance mechanism: an overview]
• [Mechanisms underlying dopaminergic neurotoxicity in ischemic cerebral damage]
• [Physiological and pathologic implications for zero-stress state of the esophagus]
• [Protein kinases and Alzheimer's disease]
• [Retroperitoneal laparoscopic unroofing of renal cyst: use of close method]
• [Risk of leukemia among medical X-ray workers in China between 1950 and 1995]
• [Studies on association between lipoprotein lipase gene polymorphisms of Pvu II site and hypertriglyceridemics in Chinese]
• [Study on automatic segmentation of color images applied to blood cells]
• [Study on high performance liquid chromatography/indirect photometric detection of fosfomycin with acridine as detection reagent]
• [Study on the application of number fluorescence density in detecting autoantibodies titer]
• [The effects of surface morphology of calcium phosphate ceramics on apatite formation in dynamic SBF]
• [Transforming growth factor beta(1) and bone morphogenetic protein 2 induce the differentiation of odontoblasts in vitro]
• [Vector propagation algorithm based ECG simulation of bundle branch block]