Optimization of dielectrophoretic DNA stretching in microfabricated devices.

Optimization of dielectrophoretic DNA stretching in microfabricated devices. Research Abstract Details 

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Optimization of dielectrophoretic DNA stretching in microfabricated devices. Abstract Text:

Kyung Eun Sung,Mark A Burns,

We have found that the surface and bulk solution properties in a microfabricated device affect the degree and probability of electrostretching of DNA molecules. Using lambda phage DNA, we found that significantly hydrophilic surfaces between the electrodes decrease the efficiency of stretching. Surfaces treated with higher silane (trimethylchlorosilane) concentrations performed better presumably due to the decreased nonspecific adsorption of DNA on these surfaces compared to their more hydrophilic counterparts. The shape and dimensions of the electrodes also affected the efficiency of stretching. Both liftoff and metal etching methods produced electrodes with random microscopic peaks along the electrode's edge and were poorly suited for stretching. Annealing the electrodes (450 degrees C for 10 min) removed most of these peaks and allowed for more controlled stretching to be obtained. We also found that thin electrodes (65 nm) gave close to a 90% success rate of DNA stretching but stretching with thick electrodes (350 nm) produced only a 20% success rate.

Optimization of dielectrophoretic DNA stretching in microfabricated devices. Publishing Authors By Initials

KE Sung,MA Burns,

For similar natural sciences: chemistry: chemistry, physical: surface properties research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties research

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MEDLINE DATE:

Optimization of dielectrophoretic DNA stretching in microfabricated devices. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Analytical chemistry

VOLUME: 78

Page Numbers: 2939-47

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 1

MONTH: May

YEAR: 2006

Optimization of dielectrophoretic DNA stretching in microfabricated devices. Information

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LANGUAGE: eng

NlmUniqueID: 370536

Optimization of dielectrophoretic DNA stretching in microfabricated devices. Keywords Mesh Terms:

KEYWORDS: Surface Properties

MESH TERMS: methods

Chemical & Substance for Abstract: Optimization of dielectrophoretic DNA stretching in microfabricated devices. Information

Substance Name: DNA, Viral

Registry Number: 0

Grant and Affiliation Information for Optimization of dielectrophoretic DNA stretching in microfabricated devices.

AFFILIATION: Department of Chemical Engineering and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109-2136, USA.

Country: United States

AGENCY: United States NHGRI

GRANT: P01-HG001984

ACRONYM: HG

MEDLINETA: Anal Chem

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