Optimization of cryopreservation of stem cells cultured as neurospheres: Comparison between vitrification, slow-cooling and rapid cooling freezing protocols.

Optimization of cryopreservation of stem cells cultured as neurospheres: Comparison between vitrification, slow-cooling and rapid cooling freezing protocols. Research Abstract Details 

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Optimization of cryopreservation of stem cells cultured as neurospheres: Comparison between vitrification, slow-cooling and rapid cooling freezing protocols. Abstract Text:

F C-K Tan,K H Lee,S S Gouk,R Magalhaes,A Poonepalli,M P Hande,G S Dawe,L L Kuleshova,Francis Chee Kuan Tan,Kong Heng Lee,Sok Siam Gouk,Raquel Magalhaes,Anuradha Poonepalli,Manoor Prakash Hande,Gavin S Dawe,Lilia L Kuleshova,

We compared cryopreservation of mammalian neural stem cells (NSCs) cultured as neurospheres by slow-cooling (1 C/min) in 10% (v/v) DMSO and cryopreservation by immersion into liquid nitrogen in ethylene glycol (EG)-sucrose solutions that support vitrification (40% (v/v) EG, 0.6 M sucrose) or that do not (37% v/v) EG, 0.6 M sucrose and 30% (v/v) EG, 0.6 M sucrose); the concentration of penetrating cryoprotectant in the last two solutions was lowered with the intention to reduce their toxicity towards NSCs. To protect against contamination a straw-in-straw technique was employed. Vitrification offered the best combination of preservation of structural integrity of neurospheres, cell viability (>96%), multipotency and karyotype. Rapid cooling in 37% (v/v) EG, 0.6 M sucrose afforded good viability but did not preserve structural integrity. Rapid cooling in 30% (v/v) EG, 0.6 M sucrose additionally reduced cell viability to 77%. Slow-cooling reduced cell viability to 65% and damaged the neurospheres. This study suggests that, in contrast to freezing, vitrification has immense potential for the cryopreservation of stem cells cultured as neurospheres or in other structured cultures.

Optimization of cryopreservation of stem cells cultured as neurospheres: Comparison between vitrification, slow-cooling and rapid cooling freezing protocols. Publishing Authors By Initials

FC Tan,KH Lee,SS Gouk,R Magalhaes,A Poonepalli,MP Hande,GS Dawe,LL Kuleshova,FC Tan,KH Lee,SS Gouk,R Magalhaes,A Poonepalli,MP Hande,GS Dawe,LL Kuleshova,

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MEDLINE DATE: 2007 Nov-Dec

Optimization of cryopreservation of stem cells cultured as neurospheres: Comparison between vitrification, slow-cooling and rapid cooling freezing protocols. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cryo letters

VOLUME: 28

Page Numbers: 445-60

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ISSN: 0143-2044

DAY: 9

MONTH: 01

YEAR: 2008

Optimization of cryopreservation of stem cells cultured as neurospheres: Comparison between vitrification, slow-cooling and rapid cooling freezing protocols. Information

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LANGUAGE: eng

NlmUniqueID: 9891832

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Grant and Affiliation Information for Optimization of cryopreservation of stem cells cultured as neurospheres: Comparison between vitrification, slow-cooling and rapid cooling freezing protocols.

AFFILIATION: Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Country: England

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MEDLINETA: Cryo Letters

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