Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI.

Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI. Research Abstract Details 

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Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI. Abstract Text:

Diane E Bovenkamp,Brian A Stanley,Jennifer E Van Eyk,

Cardiac troponin I (cTnI) is a key regulator of cardiac muscle contraction. Upon myocardial cell injury, cTnI is lost from the cardiac myocyte and can be detected in serum, in some cases with specific disease-induced modifications, making it an important diagnostic marker for acute myocardial injury. Presently, hospital laboratories use enzyme-linked immunosorbent assays to detect cTnI, but this type of analysis lacks information about modified forms of protein (degradation or phosphorylation) that may give a more specific diagnosis from either serum or biopsies. Because cardiac and serum tissues are widely used for proteomic analysis, it is important to detect these cTnI posttranslational modifications. Therefore, we have chosen to optimize the enrichment and detection of cTnI protein by IDM Affinity Bead pull-down and surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS or SELDI) analyses. By adjusting the chemical compositions of the buffers, we have retained antibody specificity and enriched for different forms of cTnI and its associated proteins.

Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI. Publishing Authors By Initials

DE Bovenkamp,BA Stanley,JE Van Eyk,

For similar macromolecular substances: polymers: biopolymers: microfilament proteins: troponin: troponin i research abstracts see: macromolecular substances: polymers: biopolymers: microfilament proteins: troponin: troponin i research

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MEDLINE DATE:

Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 357

Page Numbers: 91-102

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 3

MONTH: 12

YEAR: 2007

Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9214969

Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI. Keywords Mesh Terms:

KEYWORDS: Troponin I

MESH TERMS: metabolism

Chemical & Substance for Abstract: Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI. Information

Substance Name: Troponin I

Registry Number: 0

Grant and Affiliation Information for Optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI.

AFFILIATION: Department of Medicine, Johns Hopkins University, Baltimore, MD, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: N01-HV-28180

ACRONYM: HV

MEDLINETA: Methods Mol Biol

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