Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts.

Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts. Research Abstract Details 

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Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts. Abstract Text:

Brad J Spellberg,Mary Collins,Valentina Avanesian,Mayela Gomez,John E Edwards,Christopher Cogle,David Applebaum,Yue Fu,Ashraf S Ibrahim,

Although granulocyte transfusion is a logical, therapeutic option for neutropenic patients with refractory infections, significant technical barriers have prevented its widespread use. A novel phagocyte transfusion strategy has been developed based on activation of a human myeloid cell line HL-60. To further define the potential for HL-60 cells to recapitulate white cell transfusions, a shortened duration of activation was evaluated, facile quality control markers were defined, and the impact of low-dose irradiation on cell function was determined. Three days of activation resulted in increased cell viability and in vitro candidacidal capacity but with slightly higher cell replication compared with 7 days of activation. Cell viability and several flow cytometric measurements were accurate, quality control markers for HL-60 activation. In combination with activation, low-dose irradiation abrogated replication while sparing the candidacidal effects of the HL-60 cells. Infusion of irradiated, activated HL-60 cells improved survival of neutropenic, candidemic mice significantly. In summary, activated, irradiated HL-60 cells are microbicidal, have virtually no replicative capacity, and are safe and effective at protecting neutropenic mice against an otherwise 100% fatal candidal infection. With continued development, this strategy to recapitulate neutrophil functions has the potential to serve as an effective alternative to granulocyte transfusions.

Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts. Publishing Authors By Initials

BJ Spellberg,M Collins,V Avanesian,M Gomez,JE Edwards,C Cogle,D Applebaum,Y Fu,AS Ibrahim,

For similar surgical procedures, operative: transplantation: transplantation, heterologous research abstracts see: surgical procedures, operative: transplantation: transplantation, heterologous research

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Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of leukocyte biology

VOLUME: 81

Page Numbers: 632-41

Journal Abbreviation: J. Leukoc. Biol.

ISSN: 0741-5400

DAY: 8

MONTH: 12

YEAR: 2006

Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts. Information

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LANGUAGE: eng

NlmUniqueID: 8405628

Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts. Keywords Mesh Terms:

KEYWORDS: Transplantation, Heterologous

MESH TERMS: therapy

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Grant and Affiliation Information for Optimization of a myeloid cell transfusion strategy for infected neutropenic hosts.

AFFILIATION: Division of Infectious Diseases, Harbor-UCLA Medical Center, Torrance, CA 90502, USA. bspellberg@labiomed.org

Country: United States

AGENCY: United States NIAID

GRANT: R03 AI054531

ACRONYM: AI

MEDLINETA: J Leukoc Biol

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