Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes.

Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. Research Abstract Details 

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Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. Abstract Text:

Guangyao Liu,Hiroshi Kawaguchi,Toru Ogasawara,Yukiyo Asawa,Junji Kishimoto,Tsuguharu Takahashi,Ung-Il Chung,Hisayo Yamaoka,Hirotaka Asato,Kozo Nakamura,Tsuyoshi Takato,Kazuto Hoshi,

Since permanent cartilage has poor self-regenerative capacity, its regeneration from autologous human chondrocytes using a tissue engineering technique may greatly benefit the treatment of various skeletal disorders. However, the conventional autologous chondrocyte implantation is insufficient both in quantity and in quality due to two major limitations: dedifferentiation during a long term culture for multiplication and hypertrophic differentiation by stimulation for the redifferentiation. To overcome the limitations, this study attempted to determine the optimal combination in primary human chondrocyte cultures under a serum-free condition, from among 12 putative chondrocyte regulators. From the exhaustive 2(12) = 4,096 combinations, 256 were selected by fractional factorial design, and bone morphogenetic protein-2 and insulin (BI) were statistically determined to be the most effective combination causing redifferentiation of the dedifferentiated cells after repeated passaging. We further found that the addition of triiodothyronine (T3) prevented the BI-induced hypertrophic differentiation of redifferentiated chondrocytes via the suppression of Akt signaling. The implant formed by the human chondrocytes cultured in atelocollagen and poly(l-latic acid) scaffold under the BI + T3 stimulation consisted of sufficient hyaline cartilage with mechanical properties comparable with native cartilage after transplantation in nude mice, indicating that BI + T3 is the optimal combination to regenerate a clinically practical permanent cartilage from autologous chondrocytes.

Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. Publishing Authors By Initials

G Liu,H Kawaguchi,T Ogasawara,Y Asawa,J Kishimoto,T Takahashi,UI Chung,H Yamaoka,H Asato,K Nakamura,T Takato,K Hoshi,

For similar hormones, hormone substitutes, and hormone antagonists: hormones: thyroid hormones: thyronines: triiodothyronine research abstracts see: hormones, hormone substitutes, and hormone antagonists: hormones: thyroid hormones: thyronines: triiodothyronine research

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Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 20407-15

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 10

MONTH: 05

YEAR: 2007

Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. Keywords Mesh Terms:

KEYWORDS: Triiodothyronine

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. Information

Substance Name: Proto-Oncogene Proteins c-akt

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes.

AFFILIATION: Departments of Cartilage and Bone Regeneration (Fujisoft), and Sensory and Motor System Medicine, Faculty of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan.

Country: United States

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MEDLINETA: J Biol Chem

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