Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs).

Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs). Abstract Text:

Emily S Seo,Walter R P Scott,Suzana K Straus,John C Sherman,

We have designed, synthesised and characterised a series of template-assembled de novo four-helix bundles, each differing in the linker length between the template and the peptides. The helix is based on an earlier peptide sequence: EELLKKLEELLKKLG (first-generation sequence), which was designed to link the hydrophilic/hydrophobic interface of the helices. Increasing or decreasing the linker length by one glycine residue had a significant effect on the structure and properties of the template-assembled synthetic proteins (TASPs). Here, the effect of the linker length is further probed by linking the peptides closer to the hydrophobic face by using the second-generation sequence, AEELLKKLEELLKKG, in an effort to improve the packing between the helices and to better understand the helical bundles. The peptides were synthesised with 0-4 Gly linker residues and linked onto a cavitand template. The proteins were found to be alpha-helical, stable to guanidine hydrochloride (GuHCl) and to unfold cooperatively. However, their stabilities toward GuHCl, propensity to self-aggregate and structural specificity differed. The two-glycine variant of the second-generation series demonstrated the highest stability and most native-like character of all the mononeric TASPs in both the first- and second-generation series. The structural specificity of this two glycine variant is comparable to that of other known native-like de novo proteins. Molecular dynamics simulations showed that the two-glycine variant contains helices that are tilted with respect to the cavitand template and may account for its unique properties.

Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs). Publishing Authors By Initials

ES Seo,WR Scott,SK Straus,JC Sherman,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research

PUBMED ID PMID:

MEDLINE DATE:

Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chemistry (Weinheim an der Bergstrasse, Germany)

VOLUME: 13

Page Numbers: 3596-605

Journal Abbreviation:

ISSN: 0947-6539

DAY: 3

MONTH: 12

YEAR: 2007

Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9513783

Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs). Keywords Mesh Terms:

KEYWORDS: Structure-Activity Relationship

MESH TERMS: chemistry

Chemical & Substance for Abstract: Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs). Information

Substance Name: Guanidine

Registry Number: 113-00-8

Grant and Affiliation Information for Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs).

AFFILIATION: Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, V6T 1Z1, Canada.

Country: Germany

AGENCY: United States NCRR

GRANT: P41 RR-01081

ACRONYM: RR

MEDLINETA: Chemistry

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins TASPs Related Publications

• An argument for an olfactory thalamus.
• Approaches to the diagnosis of hepatocellular carcinoma.
• Biosynthetic characterization and chemoenzymatic assembly of the cryptophycins. Potent anticancer agents from cyanobionts.
• CRELD1 mutations contribute to the occurrence of cardiac atrioventricular septal defects in Down syndrome.
• Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis.
• Developing tools for engineering hybrid polyketide synthetic pathways.
• Does self-affirmation, cognitive processing, or discovery of meaning explain cancer-related health benefits of expressive writing?
• Endoscopic ultrasound-guided fine-needle aspiration of ascites.
• Examining subtypes of behavior problems among 3-year-old children, Part I: investigating validity of subtypes and biological risk-factors.
• Fewer driver synapses in higher order than in first order thalamic relays.
• Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor.
• HCV kinetics, quasispecies, and clearance in treated HCV-infected and HCV/HIV-1-coinfected patients with hemophilia.
• Hepatitis C virus coinfection and superinfection.
• Hydroxyquinone O-methylation in mitomycin biosynthesis.
• Interaction of glycolysis and mitochondrial respiration in metabolic oscillations of pancreatic islets.
• Mapping of the functional interconnections between thalamic reticular neurons using photostimulation.
• Metabolic coupling of dehydration and decarboxylation in the curacin A pathway: functional identification of a mechanistically diverse enzyme pair.
• Mice with GFAP-targeted loss of neurofibromin demonstrate increased axonal MET expression with aging.
• Molecular analysis of the role of tyrosine 224 in the active site of Streptomyces coelicolor RppA, a bacterial type III polyketide synthase.
• Neuropsychiatric and behavioral aspects of trisomy 21.
• On socioeconomic gradients in cancer registry data quality.
• Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs).
• Oregon Beach Monitoring Program: Bacterial exceedances in marine and freshwater creeks/outfall samples, October 2002-April 2005.
• Sexual and drug risk behaviors among women who have sex with women.
• Substrate profile analysis and ACP-mediated acyl transfer in Streptomyces coelicolor Type III polyketide synthases.
• Synthetic zeolites and other microporous oxide molecular sieves.
• T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1.
• The National Down Syndrome Project: design and implementation.
• The central role of entitativity in stereotypes of social categories and task groups.
• The prevalence and significance of occult hepatitis B virus in a prospective cohort of HIV-infected patients.