Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition.

Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition. Research Abstract Details 

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Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition. Abstract Text:

K Yanagisawa,A Kosaka,H Iwahana,M Nakanishi,S Tominaga,

Contact inhibition is a well-known phenomenon, but the details of its mechanism are poorly understood. Recently, cyclin-dependent kinase inhibitors have been studied intensively with respect to their regulatory role in the cell cycle, and of them, p27(Kip1) is particularly involved in contact inhibition. p27(Kip1) is believed to be regulated primarily through posttranscriptional mechanisms. We now report that cyclin-dependent kinase inhibitors, including p27, are regulated differently at the mRNA level during contact inhibition in murine BALB/c-3T3 fibroblasts. The mRNA expression of p15, p16, and p27 was up-regulated as the cell density increased, but, on the contrary, the mRNA level of p21(Cip1/WAF1/Sdi1) markedly decreased when the cells became confluent. The protein levels of these genes were regulated in the same way as their mRNA levels, and cyclin-dependent kinase-2 activity was markedly inhibited on density-mediated growth arrest of the cells. These results indicate that the regulation of mRNA expression of cyclin-dependent kinase inhibitors appears to contribute to their protein levels and to the arrest of cell growth through contact inhibition.

Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition. Publishing Authors By Initials

K Yanagisawa,A Kosaka,H Iwahana,M Nakanishi,S Tominaga,

For similar proteins: neoplasm proteins: tumor suppressor proteins research abstracts see: proteins: neoplasm proteins: tumor suppressor proteins research

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Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 125

Page Numbers: 36-40

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1999

Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Proteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition. Information

Substance Name: Cyclin-Dependent Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Opposite regulation of the expression of cyclin-dependent kinase inhibitors during contact inhibition.

AFFILIATION: Department of Biochemistry, Jichi Medical School, Yakushiji, Minamikawachi-machi, Tochigi 329-0498, Japan. kenyanag@jichi.ac.jp

Country: JAPAN

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MEDLINETA: J Biochem

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