Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla.

Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla. Abstract Text:

Joseph Lee,Qingsong Hu,Yasuhiro Nakamura,Xiaohong Wang,Xiaoliang Zhang,Xiaohong Zhu,Wei Chen,Qinglin Yang,Jianyi Zhang,

PURPOSE: To develop a rapid, robust, and accurate method for assessing myocardial energetics in mice and demonstrate its applicability to mouse models of acquired and genetic heart disease. MATERIALS AND METHODS: We combined surface coil localization (10-mm diameter, tunable between (1)H and (31)P, using adiabatic half-passage radiofrequency pulses) and surgery (electrocautery removal of anterior chest wall) to create an open-chest method for acquiring in vivo (31)P nuclear magnetic resonance (NMR) cardiac spectra from mice at 4.7T within 12 minutes. Normal BALB/c mice, BALB/c with myocardial infarction (MI), cardiomyocyte-restricted peroxisome proliferator-activated receptor-delta knockout (KO) (CR-PPARd(-/-)) and control loxP-flanked Ppard (Ppard(flox/flox)) mice were examined. RESULTS: The mean phosphocreatine (PCr)/adenosine triphosphate (ATP) ratios in control BALB/c mice, BALB/c MI mice, Ppard(flox/flox) mice, and PPAR-delta KO mice were 2.13 +/- 0.09 (N = 11), 1.35 +/- 0.07 (N = 9, P < 0.001 vs. BALB/c control), 1.92 +/- 0.09 (N = 5), and 1.31 +/- 0.12 (N = 5, P < 0.005 vs. Ppard(flox/flox) control), respectively. The significant depression of myocardial PCr/ATP we observed in these genetic/acquired models of heart disease was in accord with previous data from analogous large animal models. No NMR signal contamination from chamber blood or adjacent skeletal muscle was identified. CONCLUSION: This new technique provides cardiac (31)P spectra suitable for accurate quantitative analysis in a relatively short acquisition time, is suitable for terminal studies of mouse myocardial energy metabolism, and could be installed in virtually any NMR laboratory to study myocardial energetics in numerous mouse models of human heart disease.

Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla. Publishing Authors By Initials

J Lee,Q Hu,Y Nakamura,X Wang,X Zhang,X Zhu,W Chen,Q Yang,J Zhang,

For similar inorganic chemicals: isotopes: phosphorus isotopes research abstracts see: inorganic chemicals: isotopes: phosphorus isotopes research

PUBMED ID PMID:

MEDLINE DATE:

Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of magnetic resonance imaging : JMRI

VOLUME: 24

Page Numbers: 1269-76

Journal Abbreviation:

ISSN: 1053-1807

DAY: 3

MONTH: Dec

YEAR: 2006

Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9105850

Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla. Keywords Mesh Terms:

KEYWORDS: Phosphorus Isotopes

MESH TERMS: analysis

Chemical & Substance for Abstract: Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla. Information

Substance Name: Phosphocreatine

Registry Number: 67-07-2

Grant and Affiliation Information for Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla.

AFFILIATION: Department of Medicine, University of Minnesota Academic Health Center, Minneapolis, Minnesota 55455, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL71970

ACRONYM: HL

MEDLINETA: J Magn Reson Imaging

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Open-chest 31P magnetic resonance spectroscopy of mouse heart at 47 Tesla Related Publications

• A comparative study on the intercuspal occlusion among TMD patients, malocclusion patients and university students.
• Effect of retinoblastoma gene on human osteosarcoma cell line OS732.
• Enhanced antioxidant and cytoprotective abilities of vitamin E succinate is associated with a rapid uptake advantage in rat hepatocytes and mitochondria.
• Intervention by azithromycin on bleomycin-induced lung injury in rats and its mechanisms.
• Method development of adsorbed stationary phase open tubular capillary electrochromatography.
• Supercritical fluid CO2 extraction of colchicine from colchicine Autumnele liliaceae.
• Vascular endothelial growth factor gene expression in patients' ischemic skeletal muscle with diabetic foot.
• [A case of hypodermiasis with empyema]
• [Analysis of hepatitis G virus genotypes in some areas of China]
• [Autologous dendritic cells eliciting cytotoxicity of bone marrow cells against chronic granulocytic leukemia]
• [Changes of human recombination bone morphogenetic protein-2 in bone and marrow in tail suspended rats]
• [Cloning of the mouse Doc-1R gene by genomic walking]
• [Construction of recombinant adenoviruses carrying human urokinase-type plasminogen activator and its expression in hepatic stellate cells in vitro]
• [Effect of inhibition of telomerase activity on cisplatin-induced apoptosis in K562 cells]
• [Effects of electrical stimulation of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats]
• [Fatal complications in traffic accident injury]
• [Indomethacin induces apoptosis of K562 cells through activation of caspases and elevation of intracellular free calcium]
• [Infection and replication of hepatitis B virus and its clearance mechanism: an overview]
• [Mechanisms underlying dopaminergic neurotoxicity in ischemic cerebral damage]
• [Physiological and pathologic implications for zero-stress state of the esophagus]
• [Protein kinases and Alzheimer's disease]
• [Retroperitoneal laparoscopic unroofing of renal cyst: use of close method]
• [Risk of leukemia among medical X-ray workers in China between 1950 and 1995]
• [Studies on association between lipoprotein lipase gene polymorphisms of Pvu II site and hypertriglyceridemics in Chinese]
• [Study on automatic segmentation of color images applied to blood cells]
• [Study on high performance liquid chromatography/indirect photometric detection of fosfomycin with acridine as detection reagent]
• [Study on the application of number fluorescence density in detecting autoantibodies titer]
• [The effects of surface morphology of calcium phosphate ceramics on apatite formation in dynamic SBF]
• [Transforming growth factor beta(1) and bone morphogenetic protein 2 induce the differentiation of odontoblasts in vitro]
• [Vector propagation algorithm based ECG simulation of bundle branch block]