One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities.

One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Research Abstract Details 

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One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Abstract Text:

Hai Yu,Harshal A Chokhawala,Shengshu Huang,Xi Chen,Hai Yu,Harshal A Chokhawala,Shengshu Huang,Xi Chen,

Chemoenzymatic synthesis, which combines the flexibility of chemical synthesis and the high selectivity of enzymatic synthesis, is a powerful approach to obtain complex carbohydrates. It is a preferred method for synthesizing sialic acid-containing structures, including those with diverse naturally occurring and non-natural sialic acid forms, different sialyl linkages and different glycans that link to the sialic acid. Starting from N-acetylmannosamine, mannose or their chemically or enzymatically modified derivatives, sialic acid aldolase-catalyzed condensation reaction leads to the formation of sialic acids and their derivatives. These compounds are subsequently activated by a CMP-sialic acid synthetase and transferred to a wide range of suitable acceptors by a suitable sialyltransferase for the formation of sialosides containing natural and non-natural functionalities. The three-enzyme coupled synthesis of sialosides can be carried out in one pot without the isolation of intermediates. The time for synthesis is 4-18 h. Purification and characterization of the product can be completed within 2-3 d.

One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Publishing Authors By Initials

H Yu,HA Chokhawala,S Huang,X Chen,H Yu,HA Chokhawala,S Huang,X Chen,

For similar carbohydrates: polysaccharides: oligosaccharides: trisaccharides research abstracts see: carbohydrates: polysaccharides: oligosaccharides: trisaccharides research

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One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature protocols

VOLUME: 1

Page Numbers: 2485-92

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2006

One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Keywords Mesh Terms:

KEYWORDS: Trisaccharides

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Information

Substance Name: N-acetylneuraminate lyase

Registry Number: EC 4.1.3.3

Grant and Affiliation Information for One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities.

AFFILIATION: Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, California 95616, USA.

Country: England

AGENCY: United States NIGMS

GRANT: R01GM076360

ACRONYM: GM

MEDLINETA: Nat Protoc

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