Oligonucleotide microarrays: widely applied--poorly understood.

Oligonucleotide microarrays: widely applied--poorly understood. Research Abstract Details 

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Oligonucleotide microarrays: widely applied--poorly understood. Abstract Text:

Alex E Pozhitkov,Diethard Tautz,Peter A Noble,Alex E Pozhitkov,Diethard Tautz,Peter A Noble,

Microarray technology, which has been around for almost two decades, now provides an indispensable service to the biomedical research community. Soaring demand for high-throughput screening of genes potentially associated with cancer and other diseases, as well as the increased need for identifying microorganisms, have substantially opened up the application of this technology to many fields of science, including new ones such as array-based comparisons of whole genomes. Yet, despite this significant progress, the fundamental understanding of the pillars of this technology, have been largely unexplored, in particular for oligonucleotide-based microarrays. In fact, most of the current approaches for the design of microarrays are based on 'common-sense' parameters, such as guanine-cytosine content, secondary structure, melting temperature or possibility of minimizing the effects of nonspecific hybridization. However, recent experiments suggest that these are inadequate. Here we discuss these results, which challenge the basic principles and assumptions of oligonucleotide microarray technology. It is clear that more systematic physicochemical studies will be required to better understand the hybridization and dissociation behaviour of oligonucleotides.

Oligonucleotide microarrays: widely applied--poorly understood. Publishing Authors By Initials

AE Pozhitkov,D Tautz,PA Noble,AE Pozhitkov,D Tautz,PA Noble,

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Oligonucleotide microarrays: widely applied--poorly understood. Journal Published:

PUBLICATION TYPE: Review

Journal: Briefings in functional genomics & proteomics

VOLUME: 6

Page Numbers: 141-8

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ISSN: 1473-9550

DAY: 20

MONTH: 07

YEAR: 2007

Oligonucleotide microarrays: widely applied--poorly understood. Information

Number of References: 72

LANGUAGE: eng

NlmUniqueID: 101150306

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Grant and Affiliation Information for Oligonucleotide microarrays: widely applied--poorly understood.

AFFILIATION: University of Southern Mississippi, USA.

Country: England

AGENCY: United States NIDCR

GRANT: 1U01DE014955-01

ACRONYM: DE

MEDLINETA: Brief Funct Genomic Proteomic

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