Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development.

Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development. Research Abstract Details 

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Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development. Abstract Text:

Oligodendroglia and Schwann cells synthesize myelin-specific proteins and lipids for the assembly of the highly organized myelin membrane of the motor-sensory axons in the central (CNS) and peripheral nervous system (PNS), respectively, allowing rapid saltatory conduction. The isoforms of the main myelin proteins, the peripheral myelin basic isoproteins (MBP) and the integral proteolipid proteins, PLP and DM20, arise from alternative splicing. Activation of a cryptic splice site in exon III of plp leads to the deletion of 105 bp encoding the PLP-specific 35 amino acid residues within the cytosolic loop 3 of the four-transmembrane domain (TMD) integral membrane protein. To study the different proposed functions of DM20 during the development of oligodendrocytes and in myelination, we targeted the plp locus in embryonic stem cells by homologous recombination by a construct, which allows solely the expression of the DM20 specific exon III sequence. The resulting dm20(only) mouse line expresses exclusively DM20 isoprotein, which is functionally assembled into the membrane, forming a highly ordered and tightly compacted myelin sheath. The truncated cytosolic loop devoid of the PLP-specific 35 amino acid residues, including two thioester groups, had no impact on the periodicity of CNS myelin. In contrast to the PLP/DM20-deficient mouse, mutant CNS of dm20(only) mice showed no axonal swellings and neurodegeneration but a slow punctuated disintegration of the compact layers of the myelin sheath and a rare oligodendrocyte death developing with aging.

Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development. Publishing Authors By Initials

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Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Glia

VOLUME: 37

Page Numbers: 19-30

Journal Abbreviation: Glia

ISSN: 0894-1491

DAY: 9

MONTH: Jan

YEAR: 2002

Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development. Information

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LANGUAGE: eng

NlmUniqueID: 8806785

Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development. Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development. Information

Substance Name: Recombinant Proteins

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Grant and Affiliation Information for Oligodendrocytes expressing exclusively the DM20 isoform of the proteolipid protein gene: myelination and development.

AFFILIATION: Laboratory of Molecular Neuroscience, University of Cologne, Cologne, Germany.

Country: United States

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MEDLINETA: Glia

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