Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells.

Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells. Research Abstract Details 

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Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells. Abstract Text:

Hideki Mori,Toshika Fujitani,Yonehiro Kanemura,Masahiro Kino-Oka,Masahito Taya,Hideki Mori,Toshika Fujitani,Yonehiro Kanemura,Masahiro Kino-Oka,Masahito Taya,

Neural stem cells (NSCs) form cell aggregates called neurospheres during serum-free culture, and this aggregation contributes primarily to neurosphere development. In the present study, we conducted an observational examination of the cell clustering of mouse NSCs in relation to their migration potential. The cell clustering became significant from 12 h after the inoculation of NSCs during the examined culture period of 48 h. Three types of clustering were observed, that is, aggregation between two single cells, and coalescence between a cell and an aggregate and that between two aggregates. The aggregation between single cells occurred during the early phase (0-24 h after inoculation). Six hours after inoculation, the average migration rate of single cells that resulted in aggregation within the subsequent 12 h was higher than that of cells that did not aggregate. Fluorescent stainings of the cytoplasm and F-actin revealed the existence of mini podia and long podia, which were possible elements that produce the driving force for the migration of singly occurring NSCs.

Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells. Publishing Authors By Initials

H Mori,T Fujitani,Y Kanemura,M Kino-Oka,M Taya,H Mori,T Fujitani,Y Kanemura,M Kino-Oka,M Taya,

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Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bioscience and bioengineering

VOLUME: 104

Page Numbers: 231-4

Journal Abbreviation: J. Biosci. Bioeng.

ISSN: 1389-1723

DAY: 29

MONTH: Sep

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells.

AFFILIATION: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.

Country: Japan

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MEDLINETA: J Biosci Bioeng

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