Nyctalopin expression in retinal bipolar cells restores visual function in a mouse model of complete x-linked congenital stationary night blindness.

Nyctalopin expression in retinal bipolar cells restores visual function in a mouse model of complete x-linked congenital stationary night blindness. Research Abstract Details 

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Nyctalopin expression in retinal bipolar cells restores visual function in a mouse model of complete x-linked congenital stationary night blindness. Abstract Text:

Ronald G Gregg,Maarten Kamermans,Jan Klooster,Peter D Lukasiewicz,Neal S Peachey,Kirstan A Vessey,Maureen A McCall,Ronald G Gregg,Maarten Kamermans,Jan Klooster,Peter D Lukasiewicz,Neal S Peachey,Kirstan A Vessey,Maureen A McCall,

Mutations in the NYX gene that encodes the protein nyctalopin cause congenital stationary night blindness type 1. In no b-wave (nob) mice, a mutation in Nyx results in a functional phenotype that includes the absence of the electroretinogram b-wave and abnormal spontaneous and light-evoked activity in retinal ganglion cells (RGCs). In contrast, there is no morphological abnormality in the retina at either the light or electron microscopic levels. These functional deficits suggest that nyctalopin is required for normal synaptic transmission between retinal photoreceptors and depolarizing bipolar cells (DBCs). However, the synaptic etiology and, specifically, the exact location and function of nyctalopin, remain uncertain. We show that nob DBCs fail to respond to exogenous application of the photoreceptor neurotransmitter, glutamate, thus demonstrating a postsynaptic deficit in photoreceptor to bipolar cell communication. To determine if postsynaptic expression of nyctalopin is necessary and sufficient to rescue the nob phenotype, we constructed transgenic mice that expressed an EYFP-nyctalopin fusion protein on the dendritic tips of the DBCs. Immunohistochemical and ultrastructural studies verified that fusion protein expression was limited to the DBC dendritic tips. Fusion gene expression in nob mice restored normal outer and inner visual function as determined by the electroretinogram and RGC spontaneous and evoked responses. Together, our data show that nyctalopin expression on DBC dendrites is required for normal function of the murine retina.

Nyctalopin expression in retinal bipolar cells restores visual function in a mouse model of complete x-linked congenital stationary night blindness. Publishing Authors By Initials

RG Gregg,M Kamermans,J Klooster,PD Lukasiewicz,NS Peachey,KA Vessey,MA McCall,RG Gregg,M Kamermans,J Klooster,PD Lukasiewicz,NS Peachey,KA Vessey,MA McCall,

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Nyctalopin expression in retinal bipolar cells restores visual function in a mouse model of complete x-linked congenital stationary night blindness. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of neurophysiology

VOLUME: 98

Page Numbers: 3023-33

Journal Abbreviation: J. Neurophysiol.

ISSN: 0022-3077

DAY: 19

MONTH: 09

YEAR: 2007

Nyctalopin expression in retinal bipolar cells restores visual function in a mouse model of complete x-linked congenital stationary night blindness. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Nyctalopin expression in retinal bipolar cells restores visual function in a mouse model of complete x-linked congenital stationary night blindness.

AFFILIATION: Dept. of Biochemistry and Molecular Biology, Center for Genetics and Molecular Medicine, University of Louisville, Delia Baxter Building II, Room 221C, 580 S. Preston St., Louisville, KY 40202. ron.gregg@louisville.edu).

Country: United States

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MEDLINETA: J Neurophysiol

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