NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity.

NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity. Research Abstract Details 

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NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity. Abstract Text:

Lu Li,Ying Zhou,Libo Sun,Guichun Xing,Chunyan Tian,Jing Sun,Lingqiang Zhang,Fuchu He,Lu Li,Ying Zhou,Libo Sun,Guichun Xing,Chunyan Tian,Jing Sun,Lingqiang Zhang,Fuchu He,

Microtubule associated proteins are involved in regulation of microtubule dynamics. Its mutation and dysregulation result in severe consequences such as mitotic block and apoptosis. NuSAP has been reported as a microtubule associated protein, depletion of which by RNAi results in spindle deficiency and cytokinesis failure. However, its role in regulation of cell cycle and how NuSAP protein is controlled during cell cycle progression still remains unclear. Here we show that NuSAP can be ubiquitinated and degraded by APC/C-hCdh1 E3 ligase. Evolutionally conserved KEN box functions as the degron of NuSAP. Overexpression of NuSAP induces mitotic arrest and the microtubule associated domain and nuclear localization are both required for NuSAP to induce mitotic arrest. Furthermore, overexpression of NuSAP results in cells accumulation with microtubule bundling and spindle deficiency. Thus, our results give evidence for the first time that NuSAP protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity.

NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity. Publishing Authors By Initials

L Li,Y Zhou,L Sun,G Xing,C Tian,J Sun,L Zhang,F He,L Li,Y Zhou,L Sun,G Xing,C Tian,J Sun,L Zhang,F He,

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NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cellular signalling

VOLUME: 19

Page Numbers: 2046-55

Journal Abbreviation: Cell. Signal.

ISSN: 0898-6568

DAY: 14

MONTH: 06

YEAR: 2007

NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity. Information

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LANGUAGE: eng

NlmUniqueID: 8904683

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Grant and Affiliation Information for NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity.

AFFILIATION: State Key Laboratory of Proteomics, Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing Proteome Research Center, 27 Taiping Road, Beijing 100850, China.

Country: England

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MEDLINETA: Cell Signal

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