Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming.

Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming. Research Abstract Details 

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Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming. Abstract Text:

Nicholas J Hand,Thomas J Silhavy,

Induction of the toxic LamB-LacZ protein fusion, Hyb42-1, leads to a lethal generalized protein export defect. The prlF1 suppressor causes hyperactivation of the cytoplasmic Lon protease and relieves the inducer sensitivity of Hyb42-1. Since prlF1 does not cause a detectable change in the stability or level of the hybrid protein, we conducted a suppressor screen, seeking factors genetically downstream of lon with prlF1-like phenotypes. Two independent insertions in the ygdP open reading frame relieve the toxicity of the fusion protein and share two additional properties with prlF1: cold sensitivity and the ability to suppress the temperature sensitivity of a degP null mutation. Despite these similarities, ygdP does not appear to act in the same genetic pathway as prlF1 and lon, suggesting a fundamental link between the phenotypes. We speculate that the common properties of the suppressors relate to secretion defects. The ygdP gene (also known as nudH) has been shown to encode a Nudix protein that acts as a dinucleotide oligophosphate (alarmone) hydrolase. Our results suggest that loss of ygdP function leads to the induction of an alarmone-mediated response that affects secretion. Using an epitope-tagged ygdP construct, we present evidence that this response is sensitive to secretion-related stress and is regulated by differential proteolysis of YgdP in a self-limiting manner.

Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming. Publishing Authors By Initials

NJ Hand,TJ Silhavy,

For similar genetic processes: mutagenesis: suppression, genetic research abstracts see: genetic processes: mutagenesis: suppression, genetic research

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Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 185

Page Numbers: 6530-9

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 27

MONTH: Nov

YEAR: 2003

Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming. Keywords Mesh Terms:

KEYWORDS: Suppression, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming. Information

Substance Name: nudix hydrolases

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming.

AFFILIATION: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM34821

ACRONYM: GM

MEDLINETA: J Bacteriol

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