Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome.

Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Research Abstract Details 

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Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Abstract Text:

T Ohno,M Aoyagi,Y Yamanashi,H Saito,S Ikawa,T Meshi,Y Okada,

The sequence of about 4,500 nucleotides of the internal part of tobacco mosaic virus (TMV)-tomato strain (L) RNA has been newly determined using cloned cDNAs. Together with the previously determined partial sequences at both ends, the entire sequence of the 6,384 nucleotide genome has been completed. The 130K (1,115 amino acids), 180K (1,615 amino acids), 30K (263 amino acids) and coat protein (158 amino acids) cistrons are located at residues 72-3442, 72-4922, 4906-5700, and 5703-6182 on the genome, respectively. Sequence polymorphism was not observed except for heterogeneity in the length of the A cluster near the 3' end. The homology of the nucleotide sequences of TMV-L and TMV-vulgare, a common strain, is about 80% on average. Remarkable differences between them were found in a part of the N-terminal portion of the 130K/180K protein and the C-terminal portion of the 30K protein. A new method for cDNA cloning was developed by which the cDNA of the 5'-terminus of viral RNA can be cloned efficiently.

Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Publishing Authors By Initials

T Ohno,M Aoyagi,Y Yamanashi,H Saito,S Ikawa,T Meshi,Y Okada,

For similar viruses: plant viruses: mosaic viruses: tobamovirus: tobacco mosaic virus research abstracts see: viruses: plant viruses: mosaic viruses: tobamovirus: tobacco mosaic virus research

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Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 1915-23

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1984

Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Keywords Mesh Terms:

KEYWORDS: Tobacco Mosaic Virus

MESH TERMS: genetics

Chemical & Substance for Abstract: Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome.

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Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: X02144

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