Nucleotide sequence of the regulatory region of malB operons in E. coli.

Nucleotide sequence of the regulatory region of malB operons in E. coli. Research Abstract Details 

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Nucleotide sequence of the regulatory region of malB operons in E. coli. Abstract Text:

M Ohsumi,T Sekiya,S Nishimura,M Ohki,

The nucleotide sequence of a cloned section of the Escherichia coli chromosome containing the promoter regions of the malB divergent operons was determined. The region of the proximal gene, malE of the malEFG operon, was identified on the basis of the known amino acid sequence of the precursor molecule of maltose-binding protein. The region of malK, the proximal gene of the malKlamB operon, was deduced from the observation that a cloned segment contains an amino-terminal portion of the malK gene. The non-coding region between malE and malK is 299 base pairs long and contains two long GC clusters. Another feature of this region that may be related to the regulation of gene expression is the presence of two palindromic structures between the GC clusters. The DNA regions binding to cyclic AMP binding protein were determined by a method using polyacrylamide gel electrophoresis. The sites are thought to be located close to GC clusters.

Nucleotide sequence of the regulatory region of malB operons in E. coli. Publishing Authors By Initials

M Ohsumi,T Sekiya,S Nishimura,M Ohki,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Nucleotide sequence of the regulatory region of malB operons in E. coli. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 243-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1983

Nucleotide sequence of the regulatory region of malB operons in E. coli. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Nucleotide sequence of the regulatory region of malB operons in E. coli. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: genetics

Chemical & Substance for Abstract: Nucleotide sequence of the regulatory region of malB operons in E. coli. Information

Substance Name: adenosine cyclic-3',5'-monophosphate bin

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: X02038

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