Nucleotide sequence of cDNA encoding the mitochondrial precursor protein of the ATPase inhibitor from the giant panda (Ailuropoda melanoleuca).

Nucleotide sequence of cDNA encoding the mitochondrial precursor protein of the ATPase inhibitor from the giant panda (Ailuropoda melanoleuca). Research Abstract Details 

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Nucleotide sequence of cDNA encoding the mitochondrial precursor protein of the ATPase inhibitor from the giant panda (Ailuropoda melanoleuca). Abstract Text:

Wan-ru Hou,Yu-jie Du,Yu Chen,Xia Wu,Zheng-song Peng,Jun Yang,Cai-Quan Zhou,Wan-ru Hou,Yu-jie Du,Yu Chen,Xia Wu,Zheng-song Peng,Jun Yang,Cai-Quan Zhou,

Mitochondrial ATP synthase (F1Fo-ATPase) is regulated by an intrinsic ATPase inhibitor protein. In the present study, using RT-PCR combined with in silico cloning, we isolated and sequenced the cDNA encoding the inhibitor protein of the giant panda (Ailuropoda melanoleuca). The deduced protein sequence showed that the protein is composed of 106 amino acids and the estimated molecular weight of the ATPIF(1) protein is 12.32 kDa with an isoelectric point (pI) of 10.17. Alignment analysis revealed that the deduced protein sequence shares 66%, 78.3%, 66%, 72.6%, 77.4%, and 78.3% homology with that of Mus musculus, Pan troglodytes, Rattus norvegicus, Bos taurus, Macaca mulatta, and Homo sapiens, respectively. Topology prediction showed that there are three protein kinase C phosphorylation sites, one amidation site, three N-myristoylation sites, one casein kinase II phosphorylation site, and one tyrosine kinase phosphorylation site in the ATPase inhibitor. In particular, amino acids in the region between 39 and 72, which is the minimum sequence showing ATPase inhibitory activity, were highly conserved in the protein.

Nucleotide sequence of cDNA encoding the mitochondrial precursor protein of the ATPase inhibitor from the giant panda (Ailuropoda melanoleuca). Publishing Authors By Initials

WR Hou,YJ Du,Y Chen,X Wu,ZS Peng,J Yang,CQ Zhou,WR Hou,YJ Du,Y Chen,X Wu,ZS Peng,J Yang,CQ Zhou,

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Nucleotide sequence of cDNA encoding the mitochondrial precursor protein of the ATPase inhibitor from the giant panda (Ailuropoda melanoleuca). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: DNA and cell biology

VOLUME: 26

Page Numbers: 799-802

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ISSN: 1044-5498

DAY: 16

MONTH: Nov

YEAR: 2007

Nucleotide sequence of cDNA encoding the mitochondrial precursor protein of the ATPase inhibitor from the giant panda (Ailuropoda melanoleuca). Information

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LANGUAGE: eng

NlmUniqueID: 9004522

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Grant and Affiliation Information for Nucleotide sequence of cDNA encoding the mitochondrial precursor protein of the ATPase inhibitor from the giant panda (Ailuropoda melanoleuca).

AFFILIATION: College of Life Science, China West Normal University, Nanchong, Sichuan, China. hwr168@yahoo.com.cn

Country: United States

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MEDLINETA: DNA Cell Biol

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