Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus.

Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. Research Abstract Details 

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Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. Abstract Text:

H Shinkawa,Y Hatada,M Okada,H Kinashi,O Nimi,

The hrdB homologue was isolated from a streptomycin-producing Streptomyces griseus 2247 strain, which is independent of A-factor. The nucleotide sequence of the cloned DNA fragment revealed the presence of an open reading frame (ORF) of 1,542bp, which predicted a primary product of 514 amino acids and Mr 56,100. The N-terminal sequence of the purified HrdB protein of S. griseus was identical to the amino acid sequence deduced from the nucleotide sequence. The deduced amino acid sequence contains an "rpoD box" conserved in the principal sigma factors of eubacteria, and shows high similarity to the hrdB products of S. coelicolor A3(2)(89.9%) and S. aureofaciens (88.1%). The cloned gene encodes a principal sigma factor of S. griseus. The promoter region was identified by using a promoter-probe vector and by means of primer extensions experiments. The transcription start point is located 158-bp upstream of the initiation codon.

Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. Publishing Authors By Initials

H Shinkawa,Y Hatada,M Okada,H Kinashi,O Nimi,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 494-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1995

Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. Information

Substance Name: HrdB protein, Streptomyces

Registry Number: 146409-37-2

Grant and Affiliation Information for Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus.

AFFILIATION: Department of Fermentation Technology, Faculty of Engineering, Hiroshima University.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: L08071

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