Nuclear pore composition and gating in herpes simplex virus-infected cells.

Nuclear pore composition and gating in herpes simplex virus-infected cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Nuclear pore composition and gating in herpes simplex virus-infected cells. Abstract Text:

The mechanism by which herpes simplex virus (HSV) exits the nucleus remains a matter of controversy. The generally accepted route proposes that capsids exit via primary envelopment at the inner nuclear membrane and subsequent fusion of this primary particle with the outer nuclear membrane to gain capsid entry to the cytoplasm. However, recent observations indicate that HSV may induce gross morphological alterations of nuclear pores, resulting in the loss of normal pores and the appearance of dilated gaps in the nuclear membrane of up to several 100 nm. On this basis, it was proposed that a main route of capsid exit from the nucleus is directly through these altered pores. Here, we examine the biochemical composition of some of the major nuclear pore components in uninfected and HSV-infected cells. We show that total levels of major nucleoporins and their sedimentation patterns in density gradients remain largely unchanged up to 18 h after HSV infection. Some alteration in modification of one nucleoporin, Nup358/RanBP2, was observed during enrichment with anti-nucleoporin antibody and probing for O glycosylation. In addition, we examine functional gating within the nucleus in live cells, using microinjection of labeled dextran beads and a recombinant virus expressing GFP-VP16 to track the progress of infection. The nuclear permeability barrier for molecules bigger than 70 kDa remained intact throughout infection. Thus, in a functional assay in live cells, we find no evidence for gross perturbation to the gating of nuclear pores, although this might not exclude a small population of modified pores.

Nuclear pore composition and gating in herpes simplex virus-infected cells. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Nuclear pore composition and gating in herpes simplex virus-infected cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of virology

VOLUME: 82

Page Numbers: 8392-9

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 18

MONTH: 06

YEAR: 2008

Nuclear pore composition and gating in herpes simplex virus-infected cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Nuclear pore composition and gating in herpes simplex virus-infected cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Nuclear pore composition and gating in herpes simplex virus-infected cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Nuclear pore composition and gating in herpes simplex virus-infected cells.

AFFILIATION: Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom.

Country: United States

AGENCY: United Kingdom Wellcome T

GRANT:

ACRONYM:

MEDLINETA: J Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Nuclear pore composition and gating in herpes simplex virus-infected cells Related Publications

• Age of onset and outcome in Landau-Kleffner syndrome (1985).
• Anatomy of the ward round.
• Association of hepatitis C seropositivity with increased risk for developing end-stage renal disease.
• Developing a tissue perfusion sensor.
• Harm reduction in the Mersey Region.
• Identification of a highly conserved, functional nuclear localization signal within the N-terminal region of herpes simplex virus type 1 VP1-2 tegument protein.
• Improving access to Mental Health Review Tribunals.
• Individually addressable microelectrode array for monitoring oxygen and nitric oxide release.
• Letter from ireland 2007.
• Maintaining standards for pressure ulcer management in care homes.
• Merseyside, the first harm reduction conferences, and the early history of harm reduction.
• Mothers' experiences of facilitated peer support groups and individual child health nursing support: a comparative evaluation.
• Nuclear pore composition and gating in herpes simplex virus-infected cells.
• Racial differences in end-stage renal disease rates in HIV infection versus diabetes.
• Scaling in nonstationary voltammetry representations.
• Sequential clot strength analyses following diclofenac in pediatric adenotonsillectomy.
• Subacute bacterial endocarditis in pregnancy.
• Synapse-specific changes in serotonin signalling contribute to age-related changes in the feeding behaviour of the pond snail, Lymnaea.
• Synthesis, morphology, structure, and magnetic characterization of layered cobalt hydroxyisocyanates.
• Synthesis, structure, and characterization of a new thorium-organic framework material, Th(3)F(5)[(C(10)H(14))(CH(2)CO(2))(2)](3)(NO(3)).
• TOF-2: A Large 1D Channel Thorium Organic Framework.
• The expression patterns of integrin subunits on human breast tissues obtained during pregnancy.
• The impact of HIV on chronic kidney disease outcomes.
• The undergraduate and postgraduate teaching of geriatirc medicine.
• Thiazolidinediones and the renal and hormonal response to water immersion-induced volume expansion in type 2 diabetes mellitus.
• Trafficking of the bZIP Transmembrane Transcription Factor CREB-H into Alternate Pathways of ERAD and Stress-Regulated Intramembrane Proteolysis.
• Transmembrane bZIP transcription factors in ER stress signaling and the unfolded protein response.
• [(CH3)2NH(CH2)2NH(CH3)2][(UO2)2F2(HPO4)2]: a new organically templated layered uranium phosphate fluoride--synthesis, structure, characterization, and ion-exchange reactions.
• [(Th2F5)(NC7H5O4)2(H2O)][NO3]: an actinide-organic open framework.