Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules.

Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Abstract Text:

Amy J Prunuske,Jin Liu,Suzanne Elgort,Jomon Joseph,Mary Dasso,Katharine S Ullman,

When higher eukaryotic cells transition into mitosis, the nuclear envelope, nuclear pore complexes, and nuclear lamina are coordinately disassembled. The COPI coatomer complex, which plays a major role in membrane remodeling at the Golgi, has been implicated in the process of nuclear envelope breakdown and requires interactions at the nuclear pore complex for recruitment to this new site of action at mitosis. Nup153, a resident of the nuclear pore basket, was found to be involved in COPI recruitment, but the molecular nature of the interface between COPI and the nuclear pore has not been fully elucidated. To better understand what occurs at the nuclear pore at this juncture, we have probed the role of the nucleoporin Nup358/RanBP2. Nup358 contains a repetitive zinc finger domain with overall organization similar to a region within Nup153 that is critical to COPI association, yet inspection of these two zinc finger domains reveals features that also clearly distinguish them. Here, we found that the Nup358 zinc finger domain, but not a zinc finger domain from an unrelated protein, binds to COPI and dominantly inhibits progression of nuclear envelope breakdown in an assay that robustly recapitulates this process in vitro. Moreover, the Nup358 zinc finger domain interferes with COPI recruitment to the nuclear rim. Consistent with a role for this pore protein in coordinating nuclear envelope breakdown, Nup358-specific antibodies impair nuclear disassembly. Significantly, targeting either Nup153 or Nup358 for inhibition perturbs nuclear envelope breakdown, supporting a model in which these nucleoporins play nonredundant roles, perhaps contributing to COPI recruitment platforms on both the nuclear and cytoplasmic faces of the pore. We found that an individual zinc finger is the minimal interface for COPI association, although tandem zinc fingers are optimal. These results provide new information about the critical components of nuclear membrane remodeling and lay the foundation for a better understanding of how this process is regulated.

Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Publishing Authors By Initials

AJ Prunuske,J Liu,S Elgort,J Joseph,M Dasso,KS Ullman,

For similar zinc fingers research abstracts see: zinc fingers research

PUBMED ID PMID:

MEDLINE DATE:

Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular biology of the cell

VOLUME: 17

Page Numbers: 760-9

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 28

MONTH: 11

YEAR: 2005

Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Keywords Mesh Terms:

KEYWORDS: Zinc Fingers

MESH TERMS: physiology

Chemical & Substance for Abstract: Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. Information

Substance Name: ran-binding protein 2

Registry Number: 0

Grant and Affiliation Information for Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules.

AFFILIATION: Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA.

Country: United States

AGENCY: United States NICHD

GRANT: Z01 HD008740-06

ACRONYM: HD

MEDLINETA: Mol Biol Cell

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules Related Publications

• Analysis of RNA export using Xenopus oocytes.
• Assessing risk of reoffending in adolescents who have committed a sexual offense: the accuracy of clinical judgments after completion of risk assessment instruments.
• Biochemical and physiological properties of isolated platelet membranes.
• Brief report: enhanced picture naming in autism.
• Chromoendoscopy should be the standard method and more widely used for cancer surveillance colonoscopy in ulcerative colitis--con.
• Class-based feature matching across unrestricted transformations.
• Commentary.
• Comparing gang and individual rapes in a community sample of urban women.
• Discoid and subacute lupus erythematosus treated with 0.5% R-salbutamol cream.
• Disparities in inflammatory bowel disease care: time to correct a problem.
• Double dissociation between rules and memory in music: an event-related potential study.
• Dream.
• Functional characterization of nucleoside transporter gene replacements in Leishmania donovani.
• Genetic selection for a highly functional cysteine-less membrane protein using site saturation mutagenesis.
• Image interpretation by a single bottom-up top-down cycle.
• Influence of neurohumoral blockade on heart rate and blood pressure responses to haemorrhage in isoflurane anaesthetized rats.
• Leishmania donovani polyamine biosynthetic enzyme overproducers as tools to investigate the mode of action of cytotoxic polyamine analogs.
• METHENAMINE MANDELATE (MANDELAMINE) IN URINARY TRACT INFECTIONS.
• Molecular characterization of the Ran-binding zinc finger domain of Nup153.
• Not Available
• Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules.
• One thousand unsuccessful careers.
• Purine and pyrimidine metabolism in Leishmania.
• Speeded processing of grammar and tool knowledge in Tourette's syndrome.
• Structural equation modeling: reviewing the basics and moving forward.
• The aged: satisfied or dissatisfied?
• The inhibitory and necrosis-inducing effects of the latex of Ficus carica L. on transplanted and spontaneous tumours.
• Vasopressin and angiotensin II in blood pressure control during isoflurane anesthesia in rats.