Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome.

Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Research Abstract Details 

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Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Abstract Text:

Naoko Tanaka,Anna Aronova,Beate Schwer,

DEAD/H-box NTPases remodel the spliceosome at multiple steps during the pre-mRNA splicing cycle. The RNA-dependent NTPase Prp43 catalyzes dissociation of excised lariat-intron from the spliceosome, but it is unclear how Prp43 couples the energy of ATP hydrolysis to intron release. Here, we report that activation of Prp43's inherently feeble helicase activity by the splicing factor Ntr1 is required for lariat-intron release. Lethal Prp43 mutants T384A and T384V, which are active for ATP hydrolysis and fail to dissociate lariat-intron from spliceosomes, are refractory to stimulation of RNA unwinding by Ntr1. An N-terminal 120-amino-acid segment of Ntr1 suffices for binding to Prp43 and for stimulating its helicase activity. We identify missense mutations in Prp43 and Ntr1 that disrupt protein-protein interaction and impair Ntr1 enhancement of Prp43 RNA unwinding. Our results demonstrate for the first time that regulating the motor activity of a DEAH-box protein by an accessory factor is critical for mRNA splicing.

Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Publishing Authors By Initials

N Tanaka,A Aronova,B Schwer,

For similar cells: cellular structures: intracellular space: cell nucleus: cell nucleus structures: intranuclear space: spliceosomes research abstracts see: cells: cellular structures: intracellular space: cell nucleus: cell nucleus structures: intranuclear space: spliceosomes research

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Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genes & development

VOLUME: 21

Page Numbers: 2312-25

Journal Abbreviation: Genes Dev.

ISSN: 0890-9369

DAY: 15

MONTH: Sep

YEAR: 2007

Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8711660

Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Keywords Mesh Terms:

KEYWORDS: Spliceosomes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Information

Substance Name: PRP43 protein, S cerevisiae

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome.

AFFILIATION: Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM50288

ACRONYM: GM

MEDLINETA: Genes Dev

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