Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler.

Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Research Abstract Details 

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Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Abstract Text:

Hidefumi Sasaki,Katsuhiro Okuda,Osamu Kawano,Katsuhiko Endo,Haruhiro Yukiue,Tomoki Yokoyama,Motoki Yano,Yoshitaka Fujii,

Activating mutations of Ras gene families have been found in a variety of human malignancies, including lung cancer, suggesting their dominant role in tumorigenesis. Many studies have showed that the Kras gene is activated by point mutations in approximately 15-20% of non-small cell lung cancers (NSCLCs), however, there are only a few reports on Nras mutations in NSCLC. We have genotyped Nras mutation status (n=195) and Kras mutation status (n=190) in surgically treated lung adenocarcinoma cases. The presence or absence of Nras and Kras mutations was analyzed by real-time quantitative polymerase chain reaction (PCR) with mutation-specific sensor and anchor probes. EGFR mutation status at kinase domain has already been reported. Nras mutation was found in 1 of 195 patients. This mutation was a G-to-T transversion, involving the substitution of the normal glycine (GGT) with cystein (TGT) and thought to be a somatic mutation. The patient was male and a smoker. Kras mutant patients (11.1%; 21/190) had a significantly worse prognosis than wild-type patients (p=0.0013). Eighty-two EGFR mutations at kinase domain had exclusively Nras or Kras mutations. Although Nras gene mutation might be one of the mechanisms of oncogenesis of lung adenocarcinoma, this was a very rare event. Further studies are needed to confirm the mechanisms of Nras mutations for the sensitivity of molecular target therapy for lung cancer.

Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Publishing Authors By Initials

H Sasaki,K Okuda,O Kawano,K Endo,H Yukiue,T Yokoyama,M Yano,Y Fujii,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: ras proteins research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: ras proteins research

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Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Oncology reports

VOLUME: 18

Page Numbers: 623-8

Journal Abbreviation: Oncol. Rep.

ISSN: 1021-335X

DAY: 28

MONTH: Sep

YEAR: 2007

Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9422756

Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Keywords Mesh Terms:

KEYWORDS: ras Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler. Information

Substance Name: ras Proteins

Registry Number: EC 3.6.5.2

Grant and Affiliation Information for Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler.

AFFILIATION: Department of Surgery II, Nagoya City University Medical School, Nagoya 467-8601, Japan. hisasaki@med.nagoya-cu.ac.jp

Country: Greece

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MEDLINETA: Oncol Rep

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