Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis.

Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis. Abstract Text:

Yide Mei,Chongwei Xie,Wei Xie,Xu Tian,Mei Li,Mian Wu,Yide Mei,Chongwei Xie,Wei Xie,Xu Tian,Mei Li,Mian Wu,

Although camptothecin (CPT) has been reported to induce apoptosis in various cancer cells, the molecular details of this regulation remain largely unknown. In this study, we demonstrate that BH3-only protein Noxa is upregulated during CPT-induced apoptosis, which is independent of p53. In addition, we show that phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway is responsible for Noxa's induction. Luciferase assay and cAMP response element binding protein (CREB) knockdown experiments further demonstrate that CREB is involved in the transcriptional upregulation of Noxa. Moreover, blocking Noxa expression using specific small interfering ribonucleic acid (siRNA) significantly reduces the apoptosis in response to CPT, indicating that Noxa is an essential mediator for CPT-induced apoptosis. Interestingly, antiapoptotic Mcl-1 was also upregulated through PI3K/Akt signaling pathway upon CPT treatment. Using immunoprecipitation assay, Noxa was found to interact with Mcl-1 in the presence or absence of CPT. Knockdown of Mcl-1 expression by short hairpin ribonucleic acid (shRNA) was shown to potentiate CPT-induced apoptosis. Consistently, ectopic overexpression of Mcl-1 rescued cells from apoptosis induced by CPT. Cells coexpressing Noxa and Mcl-1 at different ratio correlates well with the extent of apoptosis, suggesting that the balance between Noxa and Mcl-1 may determine the susceptibility of HeLa cells to CPT-induced apoptosis.

Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis. Publishing Authors By Initials

Y Mei,C Xie,W Xie,X Tian,M Li,M Wu,Y Mei,C Xie,W Xie,X Tian,M Li,M Wu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Neoplasia (New York, N.Y.)

VOLUME: 9

Page Numbers: 871-81

Journal Abbreviation: Neoplasia

ISSN: 1476-5586

DAY: 31

MONTH: Oct

YEAR: 2007

Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100886622

Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis.

AFFILIATION: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.

Country: Canada

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Neoplasia

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis Related Publications

• Characterization of human mucin 5B gene expression in airway epithelium and the genomic clone of the amino-terminal and 5'-flanking region.
• Clinical analysis of 4 cases of juvenile granulosa cell tumor of the ovary.
• Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates.
• Effects of As(2)O(3) on BCR-ABL protein level and signal transduction in CML cells.
• Etiological analysis on the ocular fungal infection.
• Expression of mPer1 and mPer2, two mammalian clock genes, in murine bone marrow.
• Monocyte chemoattractant protein-1 mRNA in human intracranial aneurysm walls.
• Roles of kappa opioid receptors in cardioprotection against ischemia: the signaling mechanisms.
• Significance of HER-2/neu expression in ovarian epithelial tumours.
• Stimulation of airway mucin gene expression by interleukin (IL)-17 through IL-6 paracrine/autocrine loop.
• Study on some mechanism of leucite microcrystallization to reinforce dental glass ceramics.
• The clinical study of lateral orbitotomy.
• The expression, characterization and roles of macrophage colony-stimulating factor receptor in human leukemia cell lines.
• Thermal hyperalgesic effects induced by intracerebroventricular injection of interleukin-1beta in rats.
• [Arsenic exposure of residents in areas near Shimen arsenic mine]
• [Clinical study of treatment with continuous renal replacement therapy on severe hepatitis with hepatic encephalopathy]
• [Content of metals in different diameter airborne particles and effect on micronuclei formation in human lymphocytes]
• [Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome]
• [Determination of triptolide in tripterygium preparations by gradient high performance liquid chromatography]
• [Discussion on the concept problem of the specific retention volume in gas chromatography]
• [Effect of recombinant human bone morphogenetic protein-2 on the expression of osteopontin in human periodontal ligament cells]
• [Liver sinusoidal endothelial cell damage during normothermic ischemia and reperfusion of rat]
• [Oral combination chemotherapy of 5'-deoxy-5-fluorouridine (furtulon) and cyclophosphamide for advanced breast cancer]
• [Pathological features of atrial isomerism and its diagnosis by computed tomography]
• [Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2]
• [Relationship between apolipoprotein E and apolipoprotein B polymorphisms in youths with coronary heart disease]
• [Screening and identification of egg mimotope of Schistosoma japonicum]
• [Studies on immunoprotection in mice after immunization with Schistosoma japonicum 22.6 kDa recombinant protein]
• [Study of acute physiology and chronic health evaluation III and multiple organ dysfunction syndrome score in evaluating severity of intensive care units patient]
• [Study on hepatic-protective effect of "yinzhihuang" granula]