Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway.

Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Research Abstract Details 

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Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Abstract Text:

Kentaro Furukawa,Akira Yoshimi,Takako Furukawa,Yukiko Hoshi,Daisuke Hagiwara,Natsuko Sato,Tomonori Fujioka,Osamu Mizutani,Takeshi Mizuno,Tetsuo Kobayashi,Keietsu Abe,

The Aspergillus nidulans high-osmolarity glycerol response (AnHOG) pathway is involved in osmoadaptation. We found that fludioxonil, a fungicide, causes improper activation of HogA mitogen-activated protein kinase (MAPK) in A. nidulans. Here we present novel reporter systems for monitoring activation of the AnHOG pathway. The promoter region of gfdB (glycerol-3-phosphate dehydrogenase), whose expression depends on the presence of HogA, was fused to a beta-glucuronidase uidA gene (GUS) to construct the reporter, which was introduced into A. nidulans wild type and hogADelta. Increased GUS activity was detected in the wild type only when it was treated with high osmolarity or fludioxonil, while reporter activity was scarcely stimulated in the hogADelta mutant. These results indicate that the reporter activity is controlled via HogA activation. Furthermore, we present possible applications of the reporter systems in screening new antifungal compounds.

Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Publishing Authors By Initials

K Furukawa,A Yoshimi,T Furukawa,Y Hoshi,D Hagiwara,N Sato,T Fujioka,O Mizutani,T Mizuno,T Kobayashi,K Abe,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: azoles: pyrroles research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: azoles: pyrroles research

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Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bioscience, biotechnology, and biochemistry

VOLUME: 71

Page Numbers: 1724-30

Journal Abbreviation: Biosci. Biotechnol. Biochem.

ISSN: 0916-8451

DAY: 7

MONTH: 07

YEAR: 2007

Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Information

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LANGUAGE: eng

NlmUniqueID: 9205717

Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Keywords Mesh Terms:

KEYWORDS: Pyrroles

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Information

Substance Name: Glycerol

Registry Number: 56-81-5

Grant and Affiliation Information for Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway.

AFFILIATION: Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. kabe@biochem.tohoku.ac.jp

Country: Japan

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MEDLINETA: Biosci Biotechnol Biochem

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