[Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction]

[Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction] Research Abstract Details 

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[Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction] Abstract Text:

Yongde Peng,Yuebin Li,Huaidong Song,Guofeng Gao,Changhui Huang,Renming Hu,Zeguang Han,Jialun Chen,

OBJECTIVE: To investigate methodology of cloning full-length cDNA from tissues of normal adrenal gland and pheochromocytoma and predict their function. METHODS: 104 samples from normal adrenal gland and 22 samples from pheochromocytoma were examined by expressed sequence tags (EST) sequencing, bioinformatics analysis, in silico cloning, rapid amplification of cDNA ends and RT-PCR. RESULTS: Among the 126 samples of novel full-length cDNA cloning 104 were from normal adrenal gland, and 22 from pheochromocytoma (PC). Among the novel genes 50 were cloned by direct sequencing of clones, 74 by in silico cloning, and 2 by rapid amplification of cDNA ends, and the localization of chromosomes was made with UniGene searching and radiation hybrid (RH) procedure. Comparison of homology and analysis of motif or domain showed that some genes may be important functional genes, such as those coding signaling proteins, proteins associated with ion channel, enzymes involved in hormone production, important transcription factors, translation initiation factors, etc. 7 of the novel full-length cDNAs are new isoforms produced by alternative splicing. CONCLUSION: 126 novel full-length cDNAs have been cloned from adrenal tissues by large-scale EST sequencing and bioinformatic tools in a rather short time, some of which may play an important role in regulating adrenal functions and tumorigenesis of adrenal gland.

[Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction] Publishing Authors By Initials

Y Peng,Y Li,H Song,G Gao,C Huang,R Hu,Z Han,J Chen,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: p21-activated kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: p21-activated kinases research

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[Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua yi xue za zhi

VOLUME: 82

Page Numbers: 31-4

Journal Abbreviation: Zhonghua Yi Xue Za Zhi

ISSN: 0376-2491

DAY: 10

MONTH: Jan

YEAR: 2002

[Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 7511141

[Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction] Keywords Mesh Terms:

KEYWORDS: p21-Activated Kinases

MESH TERMS: genetics

Chemical & Substance for Abstract: [Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction] Information

Substance Name: Heterotrimeric GTP-Binding Proteins

Registry Number: EC 3.6.1.46

Grant and Affiliation Information for [Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction]

AFFILIATION: Shanghai Institute of Endocrinology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.

Country: China

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MEDLINETA: Zhonghua Yi Xue Za Zhi

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