Novel enzymes through design and evolution.

Novel enzymes through design and evolution. Research Abstract Details 

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Novel enzymes through design and evolution. Abstract Text:

Kenneth J Woycechowsky,Katherina Vamvaca,Donald Hilvert,

The generation of enzymes with new catalytic activities remains a major challenge. So far, several different strategies have been developed to tackle this problem, including site-directed mutagenesis, random mutagenesis (directed evolution), antibody catalysis, computational redesign, and de novo methods. Using these techniques, a broad array of novel enzymes has been created (aldolases, decarboxylases, dehydratases, isomerases, oxidases, reductases, and others), although their low efficiencies (10 to 100 M(-1) s(-l)) compared to those of the best natural enzymes (10(6) to 10(8) M(-1) s(-1)) remains a significant concern. Whereas rational design might be the most promising and versatile approach to generating new activities, directed evolution seems to be the best way to optimize the catalytic properties of novel enzymes. Indeed, impressive successes in enzyme engineering have resulted from a combination of rational and random design.

Novel enzymes through design and evolution. Publishing Authors By Initials

KJ Woycechowsky,K Vamvaca,D Hilvert,

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Novel enzymes through design and evolution. Journal Published:

PUBLICATION TYPE: Review

Journal: Advances in enzymology and related areas of molecu

VOLUME: 75

Page Numbers: 241-94, xiii

Journal Abbreviation: Adv. Enzymol. Relat. Areas Mol

ISSN: 0065-258X

DAY: 3

MONTH: 12

YEAR: 2007

Novel enzymes through design and evolution. Information

Number of References: 148

LANGUAGE: eng

NlmUniqueID: 337243

Novel enzymes through design and evolution. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Novel enzymes through design and evolution. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Novel enzymes through design and evolution.

AFFILIATION: Laboratory of Organic Chemistry, ETH-Zürich, CH-8093 Zürich, Switzerland.

Country: United States

AGENCY: United States NIGMS

GRANT: GM071126-01A1

ACRONYM: GM

MEDLINETA: Adv Enzymol Relat Areas Mol Bi

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