Novel cargo-binding site in the beta and delta subunits of coatomer.

Novel cargo-binding site in the beta and delta subunits of coatomer. Research Abstract Details 

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Novel cargo-binding site in the beta and delta subunits of coatomer. Abstract Text:

Kai Michelsen,Volker Schmid,Jutta Metz,Katja Heusser,Urban Liebel,Torsten Schwede,Anne Spang,Blanche Schwappach,Kai Michelsen,Volker Schmid,Jutta Metz,Katja Heusser,Urban Liebel,Torsten Schwede,Anne Spang,Blanche Schwappach,

Arginine (R)-based ER localization signals are sorting motifs that confer transient ER localization to unassembled subunits of multimeric membrane proteins. The COPI vesicle coat binds R-based signals but the molecular details remain unknown. Here, we use reporter membrane proteins based on the proteolipid Pmp2 fused to GFP and allele swapping of COPI subunits to map the recognition site for R-based signals. We show that two highly conserved stretches--in the beta- and delta-COPI subunits--are required to maintain Pmp2GFP reporters exposing R-based signals in the ER. Combining a deletion of 21 residues in delta-COP together with the mutation of three residues in beta-COP gave rise to a COPI coat that had lost its ability to recognize R-based signals, whilst the recognition of C-terminal di-lysine signals remained unimpaired. A homology model of the COPI trunk domain illustrates the recognition of R-based signals by COPI.

Novel cargo-binding site in the beta and delta subunits of coatomer. Publishing Authors By Initials

K Michelsen,V Schmid,J Metz,K Heusser,U Liebel,T Schwede,A Spang,B Schwappach,K Michelsen,V Schmid,J Metz,K Heusser,U Liebel,T Schwede,A Spang,B Schwappach,

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Novel cargo-binding site in the beta and delta subunits of coatomer. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of cell biology

VOLUME: 179

Page Numbers: 209-17

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 22

MONTH: Oct

YEAR: 2007

Novel cargo-binding site in the beta and delta subunits of coatomer. Information

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LANGUAGE: eng

NlmUniqueID: 375356

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Grant and Affiliation Information for Novel cargo-binding site in the beta and delta subunits of coatomer.

AFFILIATION: Zentrum für Molekulare Biologie der Universität Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.

Country: United States

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MEDLINETA: J Cell Biol

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