Novel blood-based, five-gene biomarker set for the detection of colorectal cancer.

Novel blood-based, five-gene biomarker set for the detection of colorectal cancer. Research Abstract Details 

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Novel blood-based, five-gene biomarker set for the detection of colorectal cancer. Abstract Text:

Mark Han,Choong Tsek Liew,Hong Wei Zhang,Samuel Chao,Run Zheng,Kok Thye Yip,Zhen-Ya Song,Hiu Ming Li,Xiao Ping Geng,Li Xin Zhu,Jian-Jiang Lin,K Wayne Marshall,Choong Chin Liew,Mark Han,Choong Tsek Liew,Hong Wei Zhang,Samuel Chao,Run Zheng,Kok Thye Yip,Zhen-Ya Song,Hiu Ming Li,Xiao Ping Geng,Li Xin Zhu,Jian-Jiang Lin,K Wayne Marshall,Choong Chin Liew,

PURPOSE: We applied a unique method to identify genes expressed in whole blood that can serve as biomarkers to detect colorectal cancer (CRC). EXPERIMENTAL DESIGN: Total RNA was isolated from 211 blood samples (110 non-CRC, 101 CRC). Microarray and quantitative real-time PCR were used for biomarker screening and validation, respectively. RESULTS: From a set of 31 RNA samples (16 CRC, 15 controls), we selected 37 genes from analyzed microarray data that differed significantly between CRC samples and controls (P < 0.05). We tested these genes with a second set of 115 samples (58 CRC, 57 controls) using quantitative real-time PCR, validating 17 genes as differentially expressed. Five of these genes were selected for logistic regression analysis, of which two were the most up-regulated (CDA and MGC20553) and three were the most down-regulated (BANK1, BCNP1, and MS4A1) in CRC patients. Logit (P) of the five-gene panel had an area under the curve of 0.88 (95% confidence interval, 0.81-0.94). At a cutoff of logit (P) >+0.5 as disease (high risk), <-0.5 as control (low risk), and in between as an intermediate zone, the five-gene biomarker combination yielded a sensitivity of 94% (47 of 50) and a specificity of 77% (33 of 43). The intermediate zone contained 22 samples. We validated the predictive power of these five genes with a novel third set of 92 samples, correctly identifying 88% (30 of 34) of CRC samples and 64% (27 of 42) of non-CRC samples. The intermediate zone contained 16 samples. CONCLUSION: Our results indicate that the five-gene biomarker panel can be used as a novel blood-based test for CRC.

Novel blood-based, five-gene biomarker set for the detection of colorectal cancer. Publishing Authors By Initials

M Han,CT Liew,HW Zhang,S Chao,R Zheng,KT Yip,ZY Song,HM Li,XP Geng,LX Zhu,JJ Lin,KW Marshall,CC Liew,M Han,CT Liew,HW Zhang,S Chao,R Zheng,KT Yip,ZY Song,HM Li,XP Geng,LX Zhu,JJ Lin,KW Marshall,CC Liew,

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Novel blood-based, five-gene biomarker set for the detection of colorectal cancer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical cancer research : an official journal of

VOLUME: 14

Page Numbers: 455-60

Journal Abbreviation: Clin. Cancer Res.

ISSN: 1078-0432

DAY: 18

MONTH: 01

YEAR: 2008

Novel blood-based, five-gene biomarker set for the detection of colorectal cancer. Information

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LANGUAGE: eng

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AFFILIATION: Authors' Affiliations: GeneNews Corporation, Toronto, Canada.

Country: United States

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MEDLINETA: Clin Cancer Res

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