Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes.

Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. Research Abstract Details 

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Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. Abstract Text:

Surender Khurana,James Needham,Susan Park,Bonnie Mathieson,Michael P Busch,George Nemo,Phillipe Nyambi,Susan Zolla-Pazner,Suman Laal,Joseph Mulenga,Elwyn Chomba,Eric Hunter,Susan Allen,James McIntyre,Indira Hewlett,Sherwin Lee,Shixing Tang,Elliot Cowan,Chris Beyrer,Marcus Altfeld,Xu G Yu,Anatole Tounkara,Ousmane Koita,Anatoli Kamali,Nga Nguyen,Barney S Graham,Deborah Todd,Peter Mugenyi,Omu Anzala,Eduard Sanders,Nzeera Ketter,Patricia Fast,Hana Golding,

Because increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine- from virus-induced antibodies. Most of the currently tested vaccines contain multiple viral components. As a result, many vaccine recipients give positive results in FDA-licensed HIV serodetection tests. We have identified conserved sequences in Env-gp41 and Gag-p6, which are recognized soon after infection but are not included in most HIV vaccine candidates. A new HIV serodetection assay, the HIV-SELECTEST, was established that distinguishes between vaccine-induced antibodies and seroconversion due to true HIV infections. It is important to make this assay globally relevant, because many clinical trials are conducted around the world where most HIV infections are due to non-B subtype HIV-1. Therefore, the current study examined the reactivity of plasma samples from >3,000 infections with diverse HIV subtypes worldwide. The HIV-SELECTEST performed at >99% specificity and sensitivity. Both recent and established infections with clades A, B, C, D, E, F, G, J, and CRFs were detected. Antibodies elicited by other vaccinations or infections endemic to the clinical trial sites did not react in this assay. Therefore, HIV-SELECTEST could be an important differential diagnostic tool for HIV vaccine trials, blood banks, and population screening worldwide.

Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. Publishing Authors By Initials

S Khurana,J Needham,S Park,B Mathieson,MP Busch,G Nemo,P Nyambi,S Zolla-Pazner,S Laal,J Mulenga,E Chomba,E Hunter,S Allen,J McIntyre,I Hewlett,S Lee,S Tang,E Cowan,C Beyrer,M Altfeld,XG Yu,A Tounkara,O Koita,A Kamali,N Nguyen,BS Graham,D Todd,P Mugenyi,O Anzala,E Sanders,N Ketter,P Fast,H Golding,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

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Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of acquired immune deficiency syndromes (1

VOLUME: 43

Page Numbers: 304-12

Journal Abbreviation: J. Acquir. Immune Defic. Syndr

ISSN: 1525-4135

DAY: 1

MONTH: Nov

YEAR: 2006

Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100892005

Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: immunology

Chemical & Substance for Abstract: Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. Information

Substance Name: Peptide Library

Registry Number: 0

Grant and Affiliation Information for Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes.

AFFILIATION: Division of Viral Products, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA.

Country: United States

AGENCY: United States NIMH

GRANT: MH-766767

ACRONYM: MH

MEDLINETA: J Acquir Immune Defic Syndr

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