Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros.

Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Research Abstract Details 

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Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Abstract Text:

Yan Liu,Narendra Pathak,Albrecht Kramer-Zucker,Iain A Drummond,

Epithelial tubules consist of multiple cell types that are specialized for specific aspects of organ function. In the zebrafish pronephros, multiciliated cells (MCCs) are specialized for fluid propulsion, whereas transporting epithelial cells recover filtered-blood solutes. These cell types are distributed in a ;salt-and-pepper' fashion in the pronephros, suggesting that a lateral inhibition mechanism may play a role in their differentiation. We find that the Notch ligand Jagged 2 is expressed in MCCs and that notch3 is expressed in pronephric epithelial cells. Morpholino knockdown of either jagged 2 or notch3, or mutation in mind bomb (in which Notch signaling is impaired), dramatically expands ciliogenic gene expression, whereas ion transporter expression is lost, indicating that pronephric cells are transfated to MCCs. Conversely, ectopic expression of the Notch1a intracellular domain represses MCC differentiation. Gamma-secretase inhibition using DAPT demonstrated a requirement for Notch signaling early in pronephric development, before the pattern of MCC differentiation is apparent. Strikingly, we find that jagged 2 knockdown generates extra cilia and is sufficient to rescue the kidney cilia mutant double bubble. Our results indicate that Jagged 2/Notch signaling modulates the number of multiciliated versus transporting epithelial cells in the pronephros by way of a genetic pathway involving repression of rfx2, a key transcriptional regulator of the ciliogenesis program.

Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Publishing Authors By Initials

Y Liu,N Pathak,A Kramer-Zucker,IA Drummond,

For similar proteins: fish proteins: zebrafish proteins research abstracts see: proteins: fish proteins: zebrafish proteins research

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Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Development (Cambridge, England)

VOLUME: 134

Page Numbers: 1111-22

Journal Abbreviation: Development

ISSN: 0950-1991

DAY: 7

MONTH: 02

YEAR: 2007

Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8701744

Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Keywords Mesh Terms:

KEYWORDS: Zebrafish Proteins

MESH TERMS: physiology

Chemical & Substance for Abstract: Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Information

Substance Name: mind bomb protein, zebrafish

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros.

AFFILIATION: Nephrology Division, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA.

Country: England

AGENCY: United States NIDDK

GRANT: R01 DK53093

ACRONYM: DK

MEDLINETA: Development

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