Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia.

Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia. Research Abstract Details 

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Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia. Abstract Text:

D Patschan,T Michurina,H K Shi,S Dolff,S V Brodsky,T Vasilieva,L Cohen-Gould,J Winaver,P N Chander,G Enikolopov,M S Goligorsky,

Nestin, a marker of multi-lineage stem and progenitor cells, is a member of intermediate filament family, which is expressed in neuroepithelial stem cells, several embryonic cell types, including mesonephric mesenchyme, endothelial cells of developing blood vessels, and in the adult kidney. We used Nestin-green fluorescent protein (GFP) transgenic mice to characterize its expression in normal and post-ischemic kidneys. Nestin-GFP-expressing cells were detected in large clusters within the papilla, along the vasa rectae, and, less prominently, in the glomeruli and juxta-glomerular arterioles. In mice subjected to 30 min bilateral renal ischemia, glomerular, endothelial, and perivascular cells showed increased Nestin expression. In the post-ischemic period, there was an increase in fluorescence intensity with no significant changes in the total number of Nestin-GFP-expressing cells. Time-lapse fluorescence microscopy performed before and after ischemia ruled out the possibility of engraftment by the circulating Nestin-expressing cells, at least within the first 3 h post-ischemia. Incubation of non-perfused kidney sections resulted in a medullary-to-cortical migration of Nestin-GFP-positive cells with the rate of expansion of their front averaging 40 microm/30 min during the first 3 h and was detectable already after 30 min of incubation. Explant matrigel cultures of the kidney and aorta exhibited sprouting angiogenesis with cells co-expressing Nestin and endothelial marker, Tie-2. In conclusion, several lines of circumstantial evidence identify a sub-population of Nestin-expressing cells with the mural cells, which are recruited in the post-ischemic period to migrate from the medulla toward the renal cortex. These migrating Nestin-positive cells may be involved in the process of post-ischemic tissue regeneration.

Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia. Publishing Authors By Initials

D Patschan,T Michurina,HK Shi,S Dolff,SV Brodsky,T Vasilieva,L Cohen-Gould,J Winaver,PN Chander,G Enikolopov,MS Goligorsky,

For similar proteins: nerve tissue proteins research abstracts see: proteins: nerve tissue proteins research

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Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Kidney international

VOLUME: 71

Page Numbers: 744-54

Journal Abbreviation:

ISSN: 0085-2538

DAY: 7

MONTH: 02

YEAR: 2007

Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia. Information

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LANGUAGE: eng

NlmUniqueID: 323470

Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia. Keywords Mesh Terms:

KEYWORDS: Nerve Tissue Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia.

AFFILIATION: Department of Medicine, Renal Research Institute, New York Medical College, Valhalla, New York, USA. d.patschan@gmx.de

Country: United States

AGENCY: United States NIDDK

GRANT: DK 52783

ACRONYM: DK

MEDLINETA: Kidney Int

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