Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice.

Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice. Research Abstract Details 

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Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice. Abstract Text:

Charles S Landis,Kosho Yamanouchi,Hongchao Zhou,Sankar Mohan,Namita Roy-Chowdhury,David A Shafritz,Alan Koretsky,Jayanta Roy-Chowdhury,Hoby P Hetherington,Chandan Guha,

Hepatocyte transplantation (HT) is being explored as a substitute for liver transplantation for the treatment of liver diseases. For the clinical application of HT, a preparative regimen that allows preferential proliferation of transplanted cells in the host liver and a noninvasive method to monitor donor cell engraftment, proliferation, and immune rejection would be useful. We describe an imaging method that employs the creatine kinase (CK) gene as a marker of donor hepatocytes. Creatine kinase is unique among marker genes, because it is normally expressed in brain and muscle tissues and is therefore not immunogenic. Preferential proliferation of transplanted CK-expressing hepatocytes was induced by preparative hepatic irradiation and expression of hepatocyte growth factor using a recombinant adenoviral vector. CK is normally not expressed in mouse liver and its expression by the donor cells led to the production of phosphocreatine in the host liver, permitting (31)P magnetic resonance spectroscopic imaging of liver repopulation by engrafted hepatocytes. In conclusion, this study combined a noninvasive imaging technique to assess donor hepatocyte proliferation with a preparative regimen of partial liver irradiation that allowed regional repopulation of the host liver. Our results provide groundwork for future development of clinical protocols for HT.

Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice. Publishing Authors By Initials

CS Landis,K Yamanouchi,H Zhou,S Mohan,N Roy-Chowdhury,DA Shafritz,A Koretsky,J Roy-Chowdhury,HP Hetherington,C Guha,

For similar therapeutics: biological therapy: immunotherapy: immunosuppression: transplantation conditioning research abstracts see: therapeutics: biological therapy: immunotherapy: immunosuppression: transplantation conditioning research

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MEDLINE DATE:

Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Hepatology (Baltimore, Md.)

VOLUME: 44

Page Numbers: 1250-8

Journal Abbreviation: Hepatology

ISSN: 0270-9139

DAY: 3

MONTH: Nov

YEAR: 2006

Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8302946

Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice. Keywords Mesh Terms:

KEYWORDS: Transplantation Conditioning

MESH TERMS: physiology

Chemical & Substance for Abstract: Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice. Information

Substance Name: Creatine Kinase

Registry Number: EC 2.7.3.2

Grant and Affiliation Information for Noninvasive evaluation of liver repopulation by transplanted hepatocytes using 31P MRS imaging in mice.

AFFILIATION: Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01-DK064670-01

ACRONYM: DK

MEDLINETA: Hepatology

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