Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces.

Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces. Research Abstract Details 

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Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces. Abstract Text:

C Leduc,P Ten Hove,S Park,L Vroman,J Brash,E F Leonard,

This paper reports quantification of a method for measuring amounts of protein adsorbed to a surface; the method is especially useful for revealing macroscopic spatial patterns of adsorption. The experiments tested the effectiveness of iron oxide suspensions adsorbed onto the adsorbed protein to indicate, in separate trials, the amount of either human plasma fibrinogen or human serum albumin (HSA) present on glass slides. Corresponding trials, using radioactively labeled proteins, were performed to calibrate the amount of either albumin or fibrinogen adsorbed onto similar slides out of solutions of varying bulk concentrations. The oxide deposits were quantified using a scanner and an image analysis program. The isotherms produced from the collected data indicate a continuous, monotonic correlation between light absorbed by adherent oxide and surface concentration of protein. The same correlation applies to albumin and fibrinogen when surface concentrations are expressed in weight units. These results confirm that patterns of oxide deposition correspond to patterns of protein deposition and show clearly how qualitative observations, such as those previously reported, can be made quantitative with scanning and digital image analysis.

Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces. Publishing Authors By Initials

C Leduc,P Ten Hove,S Park,L Vroman,J Brash,EF Leonard,

For similar natural sciences: chemistry: chemistry, physical: surface properties research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties research

PUBMED ID PMID:

MEDLINE DATE:

Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 7

Page Numbers: 531-8

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 1995

Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces. Keywords Mesh Terms:

KEYWORDS: Surface Properties

MESH TERMS: metabolism

Chemical & Substance for Abstract: Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces. Information

Substance Name: Fibrinogen

Registry Number: 9001-32-5

Grant and Affiliation Information for Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces.

AFFILIATION: Department of Chemical Engineering, Columbia University, New York, NY 10027, USA.

Country: NETHERLANDS

AGENCY: United States NHLBI

GRANT: HL 44535

ACRONYM: HL

MEDLINETA: J Biomater Sci Polym Ed

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