Non-SELEX selection of aptamers.

Non-SELEX selection of aptamers. Research Abstract Details 

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Non-SELEX selection of aptamers. Abstract Text:

Maxim Berezovski,Michael Musheev,Andrei Drabovich,Sergey N Krylov,

Aptamers are typically selected from libraries of random DNA (or RNA) sequences by SELEX, which involves multiple rounds of alternating steps of partitioning and PCR amplification. Here we report, for the first time, non-SELEX selection of aptamers-a process that involves repetitive steps of partitioning with no amplification between them. A highly efficient affinity method, non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM), was used for partitioning. We found that three steps of NECEEM-based partitioning in the non-SELEX approach were sufficient to improve the affinity of a DNA library to a target protein by more than 4 orders of magnitude. The resulting affinity was higher than that of the enriched library obtained in three rounds of NECEEM-based SELEX. Remarkably, NECEEM-based non-SELEX selection took only 1 h in contrast to several days or several weeks required for a typical SELEX procedure by conventional partitioning methods. In addition, NECEEM-based non-SELEX allowed us to accurately measure the abundance of aptamers in the library. Not only does this work introduce an extremely fast and economical method for aptamer selection, but it also suggests that aptamers may be much more abundant than they are thought to be. Finally, this work opens the opportunity for selection of drug candidates from libraries of small molecules, which cannot be PCR-amplified and thus are not approachable by SELEX.

Non-SELEX selection of aptamers. Publishing Authors By Initials

M Berezovski,M Musheev,A Drabovich,SN Krylov,

For similar selex aptamer technique research abstracts see: selex aptamer technique research

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Non-SELEX selection of aptamers. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 1410-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 8

MONTH: Feb

YEAR: 2006

Non-SELEX selection of aptamers. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Non-SELEX selection of aptamers. Keywords Mesh Terms:

KEYWORDS: SELEX Aptamer Technique

MESH TERMS: methods

Chemical & Substance for Abstract: Non-SELEX selection of aptamers. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Non-SELEX selection of aptamers.

AFFILIATION: Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada.

Country: United States

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MEDLINETA: J Am Chem Soc

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