Non-enzymatic covalent protein labeling using a reactive tag.

Non-enzymatic covalent protein labeling using a reactive tag. Research Abstract Details 

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Non-enzymatic covalent protein labeling using a reactive tag. Abstract Text:

Hiroshi Nonaka,Shinya Tsukiji,Akio Ojida,Itaru Hamachi,Hiroshi Nonaka,Shinya Tsukiji,Akio Ojida,Itaru Hamachi,Hiroshi Nonaka,Shinya Tsukiji,Akio Ojida,Itaru Hamachi,

We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.

Non-enzymatic covalent protein labeling using a reactive tag. Publishing Authors By Initials

H Nonaka,S Tsukiji,A Ojida,I Hamachi,H Nonaka,S Tsukiji,A Ojida,I Hamachi,H Nonaka,S Tsukiji,A Ojida,I Hamachi,

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Non-enzymatic covalent protein labeling using a reactive tag. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 129

Page Numbers: 15777-9

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 1520-5126

DAY: 5

MONTH: 12

YEAR: 2007

Non-enzymatic covalent protein labeling using a reactive tag. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Non-enzymatic covalent protein labeling using a reactive tag.

AFFILIATION: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan.

Country: United States

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MEDLINETA: J Am Chem Soc

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