NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution.

NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution. Abstract Text:

Matthew J DellaVecchia,W Keither Merritt,Ye Peng,Thomas W Kirby,Eugene F DeRose,Geoffrey A Mueller,Bennett Van Houten,Robert E London,Matthew J DellaVecchia,W Keither Merritt,Ye Peng,Thomas W Kirby,Eugene F DeRose,Geoffrey A Mueller,Bennett Van Houten,Robert E London,

UvrB is a central DNA damage recognition protein involved in bacterial nucleotide excision repair. Structural information has been limited by the apparent disorder of the C-terminal domain 4 in crystal structures of intact UvrB; in solution, the isolated domain 4 is found to form a helix-loop-helix dimer. In order to gain insight into the behavior of UvrB in solution, we have performed NMR studies on [methyl-13C]methionine-labeled UvrB from Bacillus caldotenax (molecular mass=75 kDa). The 13 methyl resonances were assigned on the basis of site-directed mutagenesis and domain deletion. Solvent accessibility was assessed based on the relaxation and chemical shift responses of the probe methyl resonances to the stable nitroxide, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL). M632, located at the potential dimer interface of domain 4, provides an ideal probe for UvrB dimerization behavior. The M632 resonance of UvrB is very broad, consistent with some degree of monomer-dimer exchange and/or conformational instability of the exposed dimer interface. Upon addition of unlabeled domain 4 peptide, the M632 resonance of UvrB sharpens and shifts to a position consistent with a UvrB-domain 4 heterodimer. A dissociation constant (KD) value of 3.3 microM for the binding constant of UvrB with the domain 4 peptide was derived from surface plasmon resonance studies. Due to the flexibility of the domain 3-4 linker, inferred from limited proteolysis data and from the relaxation behavior of linker residue M607, the position of domain 4 is constrained not by the stiffness of the linking segment but by direct interactions with domains 1-3 in UvrB. In summary, UvrB homodimerization is disfavored, while domain 4 homodimerization and UvrB-domain 4 heterodimerization are allowed.

NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution. Publishing Authors By Initials

MJ DellaVecchia,WK Merritt,Y Peng,TW Kirby,EF DeRose,GA Mueller,B Van Houten,RE London,MJ DellaVecchia,WK Merritt,Y Peng,TW Kirby,EF DeRose,GA Mueller,B Van Houten,RE London,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Journal of molecular biology

VOLUME: 373

Page Numbers: 282-95

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 2

MONTH: 08

YEAR: 2007

NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution.

AFFILIATION: Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

NMR analysis of methyl-13Cmethionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution Related Publications

• A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection.
• Acute pulmonary function response to ozone in young adults as a function of body mass index.
• Amiodarone and atrial fibrillation.
• Analyzing the structure of polypeptides in membranes by fluorescence quenching.
• Assessment of left ventricular global systolic function by transoesophageal echocardiography.
• Cerebral metabolism and mood in remitted opiate dependence.
• Comprehensive phenotypic analysis of the gut intra-epithelial lymphocyte compartment: perturbations induced by acute reovirus 1/L infection of the gastrointestinal tract.
• Corticolimbic dysregulation and chronic methamphetamine abuse.
• Determination of lysine pK values using [5-13C]lysine: application to the lyase domain of DNA Pol beta.
• Effect of ceramide N-acyl chain and polar headgroup structure on the properties of ordered lipid domains (lipid rafts).
• Effect of cigarette smoking on prefrontal cortical function in nondeprived smokers performing the Stroop Task.
• Effect of sequence hydrophobicity and bilayer width upon the minimum length required for the formation of transmembrane helices in membranes.
• Effect of the structure of lipids favoring disordered domain formation on the stability of cholesterol-containing ordered domains (lipid rafts): identification of multiple raft-stabilization mechanisms.
• Effects of cigarette smoking and abstinence on stroop task performance.
• Frontoparietal cortical activity of methamphetamine-dependent and comparison subjects performing a delay discounting task.
• GSTM1 and GSTP1 and respiratory health in asthmatic children exposed to ozone.
• Genetic polymorphisms in transforming growth factor beta-1 (TGFB1) and childhood asthma and atopy.
• Genetic variation in S-nitrosoglutathione reductase (GSNOR) and childhood asthma.
• Knockin animal models of inherited arrhythmogenic diseases: what have we learned from them?
• Mouse models of long QT syndrome.
• Myocarditis following adeno-associated viral gene expression of human soluble TNF receptor (TNFRII-Fc) in baboon hearts.
• NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solution.
• Patient education: teaching patients about wound care.
• Perioperative Beta blockades: facts and controversies.
• The control of transmembrane helix transverse position in membranes by hydrophilic residues.
• The phenyltetraene lysophospholipid analog PTE-ET-18-OMe as a fluorescent anisotropy probe of liquid ordered membrane domains (lipid rafts) and ceramide-rich membrane domains.
• The role of small molecule inhibitors for veterinary patients.
• The role of the neurobiologist in redefining the diagnosis of autism.
• Traffic exposure and lung function in adults: the Atherosclerosis Risk in Communities study.
• Working memory in cigarette smokers: comparison to non-smokers and effects of abstinence.