Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena.

Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Abstract Text:

Hema Rajaram,Shree Kumar Apte,Hema Rajaram,Shree Kumar Apte,Hema Rajaram,Shree Kumar Apte,

Heat stress caused rapid and severe inhibition of photosynthesis and nitrate reduction in nitrate-supplemented cultures of the cyanobacterium Anabaena sp. strain L-31, compared to nitrogen-fixing cultures. Anabaena strains harbour two hsp60 family genes, groEL and cpn60, respectively encoding the 59 kDa GroEL and 61 kDa Cpn60 chaperonin proteins. Of these two Hsp60 chaperonins, GroEL was strongly induced during heat stress, irrespective of the nitrogen status of the cultures, but Cpn60 was rapidly repressed and degraded in heat-stressed nitrate or ammonium-supplemented cultures. The recovery of photosynthesis, nitrate assimilation and growth in heat-stressed, nitrate-supplemented cultures were preceded by resynthesis and restoration of cellular Cpn60 levels. Glutamine synthetase activity, although adversely affected by prolonged heat stress, was not dependent on either the nitrogen status or Cpn60 levels during heat stress. Overexpression of the Cpn60 protein in the closely related Anabaena sp. strain PCC7120 conferred significant protection from heat stress to growth, photosynthesis and nitrate reduction in the recombinant strain. The data favour a role for Cpn60 in carbon and nitrogen assimilation in Anabaena.

Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Publishing Authors By Initials

H Rajaram,SK Apte,H Rajaram,SK Apte,H Rajaram,SK Apte,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Microbiology (Reading, England)

VOLUME: 154

Page Numbers: 317-25

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 4

MONTH: Jan

YEAR: 2008

Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430468

Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena.

AFFILIATION: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Microbiology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena Related Publications

• A novel potassium deficiency-induced stimulon in Anabaena torulosa.
• A novel serralysin metalloprotease from Deinococcus radiodurans.
• ADAMTS-like 2 (ADAMTSL2) is a secreted glycoprotein that is widely expressed during mouse embryogenesis and is regulated during skeletal myogenesis.
• ADAMTSL3/punctin-2, a gene frequently mutated in colorectal tumors, is widely expressed in normal and malignant epithelial cells, vascular endothelial cells and other cell types, and its mRNA is reduced in colon cancer.
• Air filter materials, outdoor ozone and building-related symptoms in the BASE study.
• An assay for macrophage-mediated regulation of endothelial cell proliferation.
• An integrative expression vector for strain improvement and environmental applications of the nitrogen fixing cyanobacterium, Anabaena sp. strain PCC7120.
• Angiogenesis in eye disease: immunity gained or immunity lost?
• Blood substitutes- the polyheme trials.
• Characterization of a response regulator protein that binds to Anabaena sp. strain L-31 kdp-promoter region.
• Cloning and overexpression of alkaline phosphatase PhoK from Sphingomonas sp. strain BSAR-1 for bioprecipitation of uranium from alkaline solutions.
• Combined photodynamic therapy and intravitreal triamcinolone for neovascular age-related macular degeneration: effect of initial visual acuity on treatment response.
• Comparative in vitro study of six carbamazepine products.
• Effect of senescence on macrophage polarization and angiogenesis.
• Interleukin-10 Promotes Pathological Angiogenesis by Regulating Macrophage Response to Hypoxia during Development.
• Interleukin-1beta-driven inflammation promotes the development and invasiveness of chemical carcinogen-induced tumors.
• Intravitreal bevacizumab for treatment of choroidal neovascularization secondary to angioid streaks.
• Intravitreal pharmacotherapy: applications in retinal disease.
• Molecular mechanisms of pancreatitis: Current opinion.
• Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena.
• O-fucosylation of thrombospondin type 1 repeats in ADAMTS-like-1/punctin-1 regulates secretion: implications for the ADAMTS superfamily.
• Pancreatic stellate cells and pancreatic cancer cells: an unholy alliance.
• Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
• Pegaptanib sodium for the treatment of age-related macular degeneration.
• Senescence regulates macrophage activation and angiogenic fate at sites of tissue injury in mice.