Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. Abstract Text:

Chenjie Xu,Keming Xu,Hongwei Gu,Xiaofen Zhong,Zhihong Guo,Rongkun Zheng,Xixiang Zhang,Bing Xu,

Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. Publishing Authors By Initials

C Xu,K Xu,H Gu,X Zhong,Z Guo,R Zheng,X Zhang,B Xu,

For similar investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, infrared: spectroscopy, fourier transform infrared research abstracts see: investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, infrared: spectroscopy, fourier transform infrared research

PUBMED ID PMID:

MEDLINE DATE:

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 3392-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 24

MONTH: Mar

YEAR: 2004

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. Keywords Mesh Terms:

KEYWORDS: Spectroscopy, Fourier Transform Infrared

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins. Information

Substance Name: Platinum

Registry Number: 7440-06-4

Grant and Affiliation Information for Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.

AFFILIATION: Department of Chemistry, Department of Physics, and Bioengineering Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China. chbingxu@ust.hk

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins Related Publications

• Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
• Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography.
• Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat.
• [Analysis of the mutations of BRCA1 in 9 familiar breast cancer patients]
• [Application of the interaction models between the polymorphism(s) of metabolic gene(s) and environmental exposure]
• [Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-kappaB]
• [Changes in the activity of platelet L-arginine/nitric oxide pathway in hypercholesterolemia patients]
• [Clinical significance of changes in insulin resistance and secretion in multiple organ dysfunction syndrome patients complicated by trauma and hemorrhage]
• [Cloning and sequencing of the genes coding for the histidine-rich protein II of Plasmodium falciparum]
• [Contrast agents in magnetic resonance imaging: development and problems]
• [Differentially expressed genes associated with cold acclimation]
• [Effects of embryonic neural stem cells and glial cell line-derived neurotrophic factor in the repair of spinal cord injury]
• [Effects of ulinastatin on interleukin-8 during one-lung ventilation in surgery]
• [Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord]
• [Mechanism of modulation of synaptic efficacy by stress and learning]
• [Opinion on hydatidosis control by immuno-prophylaxis]
• [Oral mucosa graft combined with a local turn-over flap for urethral reconstruction in various hypospadias]
• [Pharmacognostical studies on the roots of Euphorbia prolifera]
• [Pharmacological comparison between virosecurinine and securinine]
• [Phenotyping and genotyping studies in a family with the compound heterozygosity for a deletional delta beta-thalassemia and a beta-thalassemia]
• [Properties of whole-cell potassium currents in mechanically dissociated Drosophila larval central neurons]
• [Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot]
• [Retrospective analysis of effectiveness of tonsillar squamous cell carcinoma: report of 108 cases]
• [Significance of hRad17 mRNA expression in human gastric cancer]
• [Study of functional L1 retrotransposon in human type 2 diabetes susceptibility loci]
• [Study on diarrhea disease and Escherichia coli strains harboring HPI pathogenicity island of Yersinia enterolitica in Shandong province]
• [The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice]
• [The influence of postburn administration of rhGH on the host inflammatory response]
• [The transplantation of latissimus dorsi flap of the base of T shape artery with the pedicle of the thoracodorsal artery]
• [Tissue-specific expression of Na(+)-H(+) exchanger isoforms at two developmental stages of human fetus]